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EC number: 695-097-5
CAS number: 15789-90-9
The test article, methyl ionone, was tested in the bacterial reverse
mutation assay using S. typhimurium tester strains TA98, TA100, TA1535,
TA1537 and E. coli tester strain WP2uvrA in the presence and absence of
Aroclor-induced rat liver S9. The assay was performed in two phases,
using the plate incorporation method. The first phase, the preliminary
toxicity assay, was used to establish the dose range for the
mutagenicitiy assay. The second phase, the mutagenicity assay, was used
to evaluate the mutagenic potential of the test article. Dimethyl
sulfoxide was selected as the solvent of choice based on solubility of
the test article and compatibility with the target cells. The test
article was soluble in dimethyl sulfoxide at a maximum concentration of
approx. 500 mg/mL. In the preliminary assay, the maximum dose tested was
5000 ug/plate; this dose was achieved using a concentration of 100 mg/mL
and a 50 uL plating aliquot. No precipitate was observed. Toxicity was
observed at greater than or equal to 6667 ug/plate and at 5000 ug/plate
with tester strain TA100 in the absence and presence of S9 activation,
respectively. Toxicity was observed at greater than or 1000 ug/plate and
at greater than or equal to 3333 ug/plate in the absence of S9
activation with tester strains TA1535 and TA1537, resp. Based on the
findings of the toxicity assay, the maximum dose plated in the
mutagenicity assay was 5000 ug/plate. In the mutagenicity assay, no
positive response was observed. Under the conditions of this study, test
article, methyl ionone, was concluded to be negative in the Bacterial
Reverse Mutation Assay.
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