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EC number: 942-523-5
CAS number: 2156594-77-1
Table: Immobilisation rates after 24
and 48 hours of exposure
Nominal loading level of the test item [mg/L]
WSF = Water soluble fraction
USE OF NOMINAL CONCENTRATIONS
The carbon content of the test item
was determined via solid TOC measurement to be 41.8%. The percentage
related to the nominal TOC concentrations attributable to the test item
were calculated after subtraction of the background value from the
control. The TOC in the three highest loadings was in the range of 85 -
98% of the nominal values throughout the exposure period. With
decreasing loadings the TOC increased markedly in comparison to the
nominal values and values of 124 — 201% of the nominal value were
determined. Generally, the measured values were constant during the
exposure period with exception of a marked increase in the test
solutions 6.25 and 12.5 mg test item/L. The reason for the high TOC
values could not be identified. The TOC values attributable to the test
item were close to or partly below the LOQ. Assuming that the additional
C-content was caused by the test item, the TOC measurement confirmed
that the loadings were close to or above the nominal loadings during the
The 48-h EC50 value is 93 mg/L in
aquatic invertebrates (Daphnia magna).
The acute toxicity to aquatic invertebrates
was determined in a study according to OECD TG 202 and in compliance
with GLP criteria. In this study daphnids (D. magna, 20 per
concentration) were exposed to nominal loading levels of 0 (control),
6.25, 12.5, 25.0, 50.0 and 100 mg/L for 48 hours under semi-static
conditions. The TOC in the three highest loadings was in the range of 85
- 98% of the nominal values throughout the exposure period. In the two
lower loadings, the TOC increased markedly in comparison to the nominal
values and values of 124 - 201% of the nominal value were determined.
The TOC measurement confirmed that the loadings were close to or above
the nominal loadings during the test period (assuming that the
additional C-content was caused by the test item). Therefore, the effect
values are based on nominal concentrations. Immobilization was recorded
after 24 and 48 hours exposure. The water quality parameters measured at
24 and 48 h, were determined to be within the acceptable limits. The
validity criteria of the test guidelines were fulfilled. After 48 hours,
immobility was determined to be 15 and 55% in the two highest test
concentrations, whereas at the other (lower) test concentrations no
effects were observed. Based on these findings the 48-h EC50 value was
estimated to be 93.0 mg/L (95% C.L.: 82.2 - 100 mg/L).
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