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EC number: 942-523-5
CAS number: 2156594-77-1
Table: Inhibition of biomass
Nominal test item loading rate [mg/L]
Inhibition of biomass [%]
Table: Inhibition of growth rate
ADDITIONAL TEST RESULTS
- LOELR: 50.00 mg/L
- NOELR: 22.00 mg/L
- NOELR: 22.00 mg/L
REASONING FOR LACK OF DATA EVALUATION
No further data evaluation was performed.
The collected data was not sufficient to determine adequately verified
No Observed Effect Loading Rates (NOELRs). The test was repeated within
the scope of a new study under ''worst case''-conditions, i.e., without
filtration of the stock and test solutions. The repeat test was
conducted using a larger concentration range (10 different loading
rates). Herby, a superior grade study is available. The results of both
studies are comparable.
Table: Inhibition of biomass and growth rate
after 72 h exposure
Inhibition at 72 h
Growth rate [%]
ErC50 = 116 mg/L (Pseudokirchneriella
ErC10 = 67.02 mg/L (Pseudokirchneriella
Two algae studies were performed
according to OECD 201 with GLP. Since the test item is an UVCB and
poorly soluble in water, algae were exposed to Water Accommodated
Fractions (WAF) / Water Soluble Fractions (WSF): Some inconclusive
results being not statistically significant, lead to no clear No
Observed Effect Loading Rates (NOELRs) in the first study. Therefore,
the test was repeated with slight modifications: a) no filtration step
was included in the preparation of test solutions to remove the
undissolved fraction of the test substance and b) the range of the test
concentrations was extended. Especially in the low concentration range
between 0.1 - 10 mg/l additional measuring points were integrated.
Therefore, the second study can be regarded as “worst case” and was
considered as the key study.
In the key study (OECD 201 incl. GLP
(BASF SE, 2017)), freshwater algae (Pseudokirchneriella subcapitata)
were exposed to nominal rates of 0 (control), 0.1, 0.5, 1.0, 2.2, 5.0,
10, 25, 50, 100 and 220 mg/L for 72 hours under static conditions. The
measured TOC-concentrations in the test item treatments were 94.1 -
123.2 % of the nominal TOC-concentration at 0 h and 84.7 - 99.1 % after
72 h exposure. All results refer to nominal test item loading rates. The
algal biomass and growth rate were determined after 0 h, 24 h, 48 h and
72 h by the parameter chlorophyll fluorescence.
Inhibition of growth rate was observed
from approximately 50 mg/L (8.8 %) and increased in a dose-dependent
manner up 220 mg/L (97.1%). Biomass was also inhibited from
approximately 50 mg/L (28.6%), and increased in a dose-dependent manner
up to 99.8% at 220 mg/L. Based on these results, the 72-h ErL50 and 72-h
EbL50 were determined at 116 and 54.88 mg/L. The 72-h ErL10 and 72-h
EbL10 were determined at 67.02 and 27.69 mg/L, respectively.
In the study indicated as supporting
(OECD 201 incl. GLP (BASF SE, 2017)), organisms were exposed under
similar conditions. Exponentially growing freshwater algae
(Pseudokirchneriella subcapitata) were exposed to nominal rates of 0
(control), 6.26, 12.5, 25, 50, 100 and 200 mg/L for 72 hours under
static conditions. The measured TOC-concentrations in the test item
treatments were 83.7 - 102.1 % of the nominal TOC-concentration at 0 h
and 54.9 - 88.2 % at 72 h exposure. The algal biomass and growth rate
were determined after 0 h, 24 h, 48 h and 72 h by the parameter
Since slight inconsistencies in the
growth rate were determined being statistically insignificant, no
adequately verified No Observed Effect Loading Rates (NOELRs) could be
determined: e.g. growth rate was positively influenced (8 %,
statistically insignificant) at 6.26 mg/l and at 12.5 mg/l no
influencing effect was observed. In order to clarify, if inconsistencies
were caused by artefacts or by effects of the test substance the test
was repeated as described previously.
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