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EC number: 269-943-5
CAS number: 68391-31-1
data for the test systems
historical data are based on results which were obtained by using the
pre-incubation method is
more sensitive than the plate incorporation method. Therefore slightly
lower mean number of revertants could be expecte d for the plate
data of S. typh.TA98: Revertant colonies
Solvent control (- S9)
11 - 40
Solvent control (+ S9)
16 - 45
Pos. control (- S9),
2-Nitrot1uorene, 0.12 µg/plate
Pos. control (+ S9),
2-Aminofluorene, 10 µg/plate
data of S. typh. TA100: Revertant colonies
88 - 123
Sodium azide, 1.0 µg/plate
434 - 2633
data of S. typh. TA102: Revertant colonies
179 - 283
Mitomycin C, 0.1 µg/plate
347 - 1133
402 - 967
= standard deviation
data of S. typh. TA1535: Revertant colonies
7 - 56
9 - 56
Sodium azide, 1 µg/plate
2-Aminoanthracene, 10 µg/plate
52 - 477
data of S. typh. TA1537: Revertant colonies
6 - 13
9 - 24
9-Arninoacridine, 2.5 µg/plate
2-Aminofluorene, 50 µg/plate
51 - 93
The test item C
chloride) was assessed for the
potential to induce genmutation in the salmonella typhimurium reverse
The test reported here was performed
according to the OECD Guideline TG 471 for the bacterial reverse
mutation test. Following bacterial strains have been used in this study:
S. typhimurium TA98, TA100, TA102, TA1535 and TA1537. In the first
experiment of the main test exposure of the test system has been
performed using the plate incorporation method. In the second experiment
the pre-incubation method was adopted.
The test item C
chloride) was applied at
concentrations of 1, 0.316, 0.1, 0.0316, 0.01 and 0.00316 mg/plate (both
experiments) with and without metabolic activation (S9 rat liver: 2
Depending on the bacterial strain,
precipitates and/or cytotoxicity was observed in higher concentrations.
Therefore, according to the OECD Guideline TG 471 the tested
concentration range was justified.
The respective negative controls produced a
low number of revertants.
All but one strain-specific positive
controls were shown to produce the expected increase in revertant
colonies, with or without metabolic activation, respectively. The
failure of 9- Aminoacridine (without S9) in the 1st experiment was
negligible, as the functionality of the strain TA1537 to indicate
genmutations was confirmed by 2-Aminofluorene (with S9) in the same
In the second experiment all strain-specific
positive controls were shown to produce the expected increase in
revertant colonies, with or without metabolic activation, respectively.
Both experiments showed no significant
inerease in the number of revertant colonies independent of metabolic
activation - in any bacteria strain. Furthermore no dose-effect
relationship could be determined.
Taken together, no mutagenic potential of
the test item C
chloride) w as determined under the
conditions of this test.
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