3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result using OECD guidelines.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Principles of method if other than guideline:

Biodegradation study was conducted according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test) for evaluating the percentage biodegradability of test substance 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,Ntrimethylanilinium chloride (CAS No 68391-31-1) .

GLP compliance:

no

Oxygen conditions:

not specified

Inoculum or test system:

other: Mixed inoculum

Details on inoculum:

Mixed Inoculum Preparation:Polyseed were used for this study. 1 polyseed capsule was added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.

Duration of test (contact time):

28 d

Initial conc.:

4 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: OECD mineral medium was used for the study
- Test temperature: 20°C
- Continuous darkness: Yes
- Other: The water used in this study is deionized water.

TEST SYSTEM
- Culturing apparatus: The apparatus used in this study is BOD bottles; with glass stoppers (125 ml), BOD incubator & oxygen electrode and meter.

CONTROL AND BLANK SYSTEM
- Inoculum blank: it contains only test inoculum
- Procedure control: contains reference compound and inoculum

Reference substance:

other: Sodium Benzoate

Key result

Parameter:

% degradation (O2 consumption)

Value:

40.69

Sampling time:

28 d

Remarks on result:

other: Other details not known

Details on results:

The oxygen consumed by the test systems was corrected for oxygen consumption occurring in the blank test systems. The BOD Values (mgO2/mg) and percent biodegradation results for each test system are reported in Tables 2 and 3, respectively. The BOD28 value of 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride (CAS No.68391-31- 1) was observed to be 0.7 mgO2/mg. ThOD was determined by calculation as 1.72 mgO2/mg. % Degradation was calculated using the values of BOD and ThOD for test item and was found to be 40.69% at 20 ± 1°C. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 78.31%. Degradation of Sodium benzoate exceeds 13.25% on 7 days & 61.44% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid

Results with reference substance:

The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 78.31%. Degradation of Sodium benzoate exceeds 13.25% on 7 days & 61.44% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid

TABLE 1:D.O VALUES(mg/L)

No. of Days	Inoculum Blank (Control)	Test Suspension	Procedure Control (Reference Item)
0	7.3	6.9	7
7	7	5.6	5.8
14	6.8	4.1	2.4
21	6.4	3.8	1.2
28	6.2	3	0.7

 

TABLE 2:BOD VALUES (mgO2/mg)

No. of Days	Test Suspension	Procedure Control (Reference Item)
0	0	0
7	0.25	0.22
14	0.57	1.02
21	0.55	1.22
28	0.7	1.3

 

TABLE 3:PERCENT BIODEGRDATION RESULTS

No. of Days	Test Suspension	Procedure Control (Reference Item)
0	0%	0%
7	14.53%	13.25%
14	33.13%	61.44%
21	31.97%	73.49%
28	40.69%	78.31%

 

TABLE 4:BOD28, THOD AND % BIODEGRADATION VALUES

Method details	BOD28 (mgO2/mg)	ThOD (mgO2/mg)	% Biodegradation
Test Item	0.7	1.72	40.69
Reference Item	1.3	1.66	78.31

 

Table 5: pH OF CLOSED BOTTLE TEST

No. of Days	Inoculum Blank (Control)	Test Suspension	Procedure Control (Reference Item)
0	6.5	7.6	7.9
7	6.6	6.6	5.8
14	6.7	6.4	6.7
21	6.2	6.3	6.2
28	6.2	6.3	6.5

 

Validity criteria fulfilled:

yes

Interpretation of results:

other: ultimate inherently biodegradable

Conclusions:

The 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride(CAS No.68391-31-1) undergoes 40.69% biodegradation after 28 days in the test condition. Thus, the test item was considered to be ultimate inherently biodegradable in nature.

Executive summary:

28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl- 1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride, CAS No. 68391-31-1. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28 value of 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,Ntrimethylanilinium chloride, CAS No. 68391-31-1 was observed to be 0.7 mgO2/mg. ThOD was calculated as 1.72 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 40.69%. Based on the results, the test item, under the test conditions, was considered to be ultimate inherently biodegradable.

 

Description of key information

The 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride(CAS No.68391-31-1) undergoes 40.69% biodegradation after 28 days in the test condition. Thus, the test item was considered to be ultimate inherently biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Additional information

28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl- 1H-pyrazol-4-yl)azo]-N,N,N-trimethylanilinium chloride, CAS No. 68391-31-1. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28 value of 3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-N,N,Ntrimethylanilinium chloride, CAS No. 68391-31-1 was observed to be 0.7 mgO2/mg. ThOD was calculated as 1.72 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 40.69%. Based on the results, the test item, under the test conditions, was considered to be ultimate inherently biodegradable.