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EC number: 213-860-9
CAS number: 1038-28-4
Table 1: Effects on behaviour of Zebra fish (n=1 0), exposed to ZK 47.569
The purpose of this study was to determine the acute toxicity/96 hours
of the test compound
Ethyldienol to the Zebra fish (Brachydanio rerio). The study was
conducted in agreement with
the test guideline of the OECD, no. 203 "Fish, Acute Toxicity Test".
Ten Zebra fish each were exposed to four concentrations of the test
substance and the dilution water for aperiod of 48 hours under
flow-through conditions. The test solutions were prepared using 0.2 g
Tween 80/L as a vehicle. The same vehicle
concentration was used for the control.
The following nominal test concentrations were selected: 1.0, 2.0, 3.6
and 8.1 mg/L. No chemical analysis was performed. However, it is assumed
that under the given flow-through conditions > 80% of the nominal
concentration of test compound was maintained taking in account the
flow-rate and the hydrolytical stability.
Mortalities and visual abnormalities were recorded at 3, 6, 24, and 48
The results of the test after 48 hours could not be evaluated due to a
technical defect of the
test system. Therefore, only the data related to the 24-hour exposure
time are reported.
Mortality (one fish) was observed in the test concentration of 3.6 mg/L
within 24 hours.
A concentration- and time-dependent occurrence of total apathy was noted
of 2.0 to 8.1 mg/L. Additionafly, two fish were found to have lost
equilibrium in the concentration
of 3.6 mg/L. The fish in the control group were free from abnormal
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