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EC number: 213-860-9
CAS number: 1038-28-4
Table 1: Biological degradation (cumulative) in percent (corrected tor
blank C02 production)
The purpose of this study was to determine the ready biodegradability of
Ethyldienol (ZK 47569). The study
was conducted in agreement with the following test guideline: OECD
guideline tor testing of chemicals, Ready biodegradability:
C02-evolution test, no. 301B.
The test substance was incubated in an aqueous solution including
nutrients with microorganisms trom a municipal sewage treatment plant
for 27 days (start of treatment = day 1). The nutrient solutions were
made up of phosphates, ammonium sulphate,
magnesium sulphate, iron chloride, ammonium chloride and calcium
chloride, and added to the test solution.
The test substance was incubated in a concentration of 10 mg carbon/L in
Additionally, a reference substance (sodium acetate) was tested
according to the same
procedure, in order to verity the viability and activity of the
Furthermore, a blank contral was tested in triplicate without any test
or reference substance.
One further set was incubated with sodium acetate as 10 mg, carbon/L
plus test substance as 10 mg carbon/L representing toxicity control.
The biological degradation of the test and reference substances was
measurement of the carbon dioxide (C02) produced during the test period.
was determined on days 4, 7, 10, 14, 18, 23 and 29, and calculated as
the percentage of total
CO2 that the test material could theoretically have produced, based on
carbon content. The
blank CO2 production was subtracted for correction.
The test compound was practically not degraded.
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