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EC number: 213-860-9
CAS number: 1038-28-4
Table 1: Mean values of turbidity (expressed as TE/F) and growth
inhibition of Pseudomonas putida as a function of the
concentration (n=3) of ZK 47569
The purpose of this study was to determine the growth inhibition of
Pseudomonas putida under
exposure to Ethyldienol. The test was conducted in agreement with the
DIN standard 38412, L8,
"Wachstumshemmtest mit dem Bakterium Pseudomonas putida".
200 mg of the test substance were suspended in 1000 ml demineralized
water under constant stirring tor
24 hours. In order to prepare the test solutions, this suspension was
filtered. This solution was
used as the highest test concentration and was further diluted 1 :2.5, 1
:5, 1: 10, 1 :20, 1 :40, 1 :80
and 1 :160. Additionally, one control without the test substance was
used. All test solutions including the control were incubated in
triplicate. Furthermore, for each test concentration one test vessel was
incubated without addition of the
inoculum in order to analyse the inherent turbidity of the test compound.
The concentrationof the saturated, filtered solution was estimated by a
subsequent calculation based on the molecular formula.
According to the results of a TOC-analysis, the saturated, filtered
solution has a concentration of < 1 mg/L.
As a parameter for the growth of the bacterial population, theturbidity
of the test and control
solutions was analysed photometrically at a wave-Iength of 436 nm.
The measurements showed a growth decrease in all solutions except the 1
:160 dilution in
comparison with the control group.
However, the control growth exceeded the required minimum of the DIN
standard only by
3% and therefore, it is doubtful, whether the observed reduction of
growth was compound related,
since it was not concentration-related at dilutions of 1:10 and lower.
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