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EC number: 213-860-9
CAS number: 1038-28-4
The purpose of this study was to determine the acute toxicity
(immobilization, EC50/48 hours) of
Ethyldienol with Daphnia magna in order to evaluate the aquatic
toxicity. Ethyldienol is an
intermediate of the synthesis of ZK 13664 (Norgestrel).
The test compound was hydrolytically unstable (half-life 27.8 hours/pH
7). The study
was conducted in a definitive test (under semi-static conditions) in
agreement with the guideline of the OECD, no. 202.
20 juvenile Daphnia magna were used for each of the test solutions of
the test compound and 20 for the
tap water control. The Daphnia were exposed to the test solutions and
the dilution water for a
period of 48 hours under semi-static conditions (the control and test
solutions were renewed 24
hours after start of exposure). Immobilization was recorded at 24 hours
and 48 hours.
For the preparation of the test solutions, a suspension with a nominal
loading of 100 mg/L was
stirred for 24 hours. This suspension was filtered through a glassfibre
filter. The resulting
solution served as the highest concentration (saturated solution). It
was further diluted 1 :2, 1:4
and 1 :8. 24 hours after start of exposure the test solutions were
The chemical analysis of the saturated solution by HPLC showed
unexpectedly low concentrations of 6.5 x 10-5 mg/L. Additionally, TOC
analysis was performed at 0, 24 and 48 hours. The mean TOC concentration
of the saturated solution was 2.4 mg/L corresponding to 2.96 mg/L of
test compound. The water solubility of Ethyldienol was determined with 6
mg/L in a standard
test. Therefore, the concentration of Ethyldienol extrapolated from TOC
analysis was considered representative.
Statistical evaluation regarding the observations of immobilization was
performed by probit analysis on the basis of the organic carbon content.
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