Registration Dossier
Registration Dossier
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EC number: 200-753-7 | CAS number: 71-43-2
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-guideline experimental study with clearly reported methods and results. Adequate for evaluation.
Data source
Reference
- Reference Type:
- publication
- Title:
- Ortho-quinones of benzene and estrogens induce hyperproliferation of human peripheral blood mononuclear cells
- Author:
- Chakravarti D, Zahid M, Backora M, Myers EM, Gaikwad N, Weisenburger DD, Cavalieri EL, Rogan EG and Joshi SS
- Year:
- 2 006
- Bibliographic source:
- Leukemia and Lymphoma 47, 2635-2644
Materials and methods
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Benzene
- EC Number:
- 200-753-7
- EC Name:
- Benzene
- Cas Number:
- 71-43-2
- Molecular formula:
- C6H6
- IUPAC Name:
- benzene
- Details on test material:
- Name of test materials (as cited in study report): benzene, benzene ortho-quinone
Constituent 1
Results and discussion
Any other information on results incl. tables
BENZENE ORTHOQUINONE ADDUCT FORMATION
Both CAT-4-N7Gua (2.3 pmol/10^6 cells) and CAT-4-N3Ade adducts (0.6 pmol/10^6 cells) were detected when MNCs were treated with 75 uM benzene ortho-quinone.
QUINONE-INDUCED HYPERPROLIFERATION
Graphical data indicate that benzene ortho-quinone significantly increased the population of mitogen-induced MNCs following 7, 14 or 21 d treatment, with treatments of 25-100 uM being generally more effective at stimulating cell proliferation than 150 uM benzene ortho-quinone. The overall recovery of cells decreased during the course of the experiments (being especially reduced by day 21) however a stimulatory response to the ortho-quinone was still apparent.Applicant's summary and conclusion
- Conclusions:
- Benzene ortho-quinone stimulates proliferation of human peripheral blood mononuclear cells in vitro.
- Executive summary:
The effect of benzene ortho-quinone (0-150 uM) on cell proliferation and DNA adducts formation in human mononuclear cells was investigated in vitro. Cell proliferation was significantly increased following 7, 14 or 21 d treatment although the extent of stimulation decreased with time. Both Catechol-4-N7Gua and catechol-4-N3Ade adducts were detected in cells exposed to 75 uM benzene ortho-quinone. The authors suggest that DNA damage induced by benzene ortho-quinone may promote growth of human blood mononuclear cells.
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