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EC number: 217-461-0
CAS number: 1860-26-0
The substance is acutely not toxic to
algae up to the maximum water solubility.
In a GLP guideline study following OECD
Guideline 201 green algae (Desmodesmus subspicatus) were exposed for 72
hours to a saturated solution of tris-2 -ethylhexylamine. This is a
limit test with a nominal concentration of 100 mg/L. the algal biomass
was determined by measuring the fluorescence of the treatments and the
control. The correlation between algal biomass and fluorescence was
determined via counting of the cell number in a dilution series of an
algal suspension (Desmodesmus subspicatus). The calibration curve has a
high correlation coefficient of R² = 0.9957, which proves the good
correlation of cell number and fluorescence.
The test solutions were prepared
following general guidance provided in OECD 23 in order to generate a
saturated solution of the test substance in test medium. Undissolved
test material was not removed. Hence, the algae were exposed to the
total load of the test substance. The dissolution behavior was observed
during exposure in uninoculated test assays. The control assays were
clear and did not show any turbidity. At the start of exposure (0 h),
the test substance was homogeneously dispersed in water showing a slight
turbidity. After 24 h, 48 h, and 72 h, undissolved test substance was
observed in the water surface as oily drops in addition to the previous
An analytical method by LC/MS for the
determination of the test substance was developed at the Analytical
Chemistry Laboratory of Experimental Toxicology and Ecology of BASF SE,
Ludwigshafen, Germany. However, an analytical verification of the test
material concentration is not feasible as the limit of quantitation
(LOQ) is significantly higher than the water solubility of the
Substance. The LOQ for this method was approx. 0.3 mg/L and the water
solubility of the test substance is < 0.002 mg/L. No reliable method for
analyses in the required concentration range could be developed.
Therefore, concentration control
analysis was not performed. However, since all reasonable efforts were
taken to produce a saturated solution of the test substance in test
media, following the guidance in OECD 23, the test results should be
based on loading concentration. The daphnids were exposed to the entire
loaded mass of test substance over each renewal period.
A study on the stability of the substance
in this algal growth inhibition test was also not performed due to the
lack of a reliable analytical method. With regard to the stability a
decline in the concentration could be possible by adsorption based on
the high log Kow. However, the substance is not biodegradable and is not
expected to degrade by hydrolysis. As undissolved test material was not
removed from the test solution, the dissolved concentration of the
Substance should still remain stable as the undissolved test material
could have served as a reservoir to compensate for any losses.
Overall, no adverse effects on the
test organisms were observed. Based on nominal concentrations, the 72-h
ErC50 for effects on algae growth rate is > 100 mg/L (nominal (loading),
BASF SE, 2010).
Therefore, it can be concluded that
the substance is acutely not harmful to algae up to the maximum water
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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