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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

The lethal concentration (LC50) value of 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid in fish[ Pimephales promelas] in a 96 hr study on mortality effect was found to be 245.685mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance , it is concluded that 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid shall not exhibit short term toxicity to fish ( Pimephales promelas ).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
245.685 mg/L

Additional information

The lethal concentration (LC50) value of phthalylsulfathiazole in fish (Pimephales promelas) in a 96 hr study on mortality effect was estimated to be 245.685mg/L. The supporting studies also predicted an LC50 value above 100 mg/L as has been mentioned in the table attached below;

Sr. No

Endpoint name





LC50 245.685 mg/l Pimephales promelas
 2  LC50  152.0679  mg/l  Oncorhynchus mykiss
 3  LC50  147.8293  mg/l  Lepomis macrochirus

As can be observed from the above information, the endpoint varies from 147.8293 to 245.685 mg/L. Thus, all the LC50 values are above 100 mg/L, so indicating that no aquatic toxicity to the fishes by 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid will be applicable. Therefore it can be concluded that the substance is not classified as per the CLP criteria for fish toxicity.