Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Saturated test solutions with 100 mg TN-105 per litre were prepared and filtered. This nominal loading of 100 mg/l was then diluted by a factor of two to get the further nominal loadings loadings of 6.25, 12.5, 25, and 50 mg/l.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
21 d

Test conditions

Nominal and measured concentrations:
Nominal loading 100 mg/L: 7.5, 3.8 and 12.4 mg/l (mean: 7.9 mg/l).
Nominal loading 50 mg/L: 3.7, 1.9 and 6.1 mg/l (mean: 3.85 mg/l)

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
other: NOEL
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
TN-105 (based on diluted extracted loading)
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
3.85 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Remarks:
TN-105 (the soluble ligand only is measurable, extrapolated to test material)
Basis for effect:
reproduction

Applicant's summary and conclusion

Conclusions:
Nominal loadings up to and including 100 mg/l did not affect survival/viability). Adult growth (body length), mean cumulative reproduction, age to first brood and intrinsic rate was unaffected up to and including a nominal loading of 50 mg/l (relevant NOEL: 50 mg/l), corresponding to a measured concentration (NOEC) of 3.85 mg/l.