Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study planned
Study period:
From Dec.1,2011 to May.1,2012

Data source

Materials and methods

GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
(1-methylethylidene)di-4,1-phenylenetetraphenyl diphosphate
Details on test material:
Same with the substance notified in the section 1 of the dossier

Sampling and analysis

Analytical monitoring:

Test substrate

Details on preparation and application of test substrate:
A mixture comprised of 10 g of finely ground industrial quartz sand with a quantity of the test substance necessary to achieve the test concentration in the soil is prepared. The mixture is then mixed thoroughly with the pre-moistened artificial soil. De-ionised water is then added to an amount required to achieve a final moisture content of 40 to 60% of the maximum water holding capacity is then added and mixed in. The soil is then ready for placing to the test containers.

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
Details on test organisms:
The species used in the test is Eisenia fetida. Adult worms between two months and one year old and with a clitellum are required to start the test. The worms were selected from a synchronised culture with a relatively homogeneous age structure. Individuals in a test group did not differ in age by more than 4 weeks.

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
Total exposure duration:
8 wk

Test conditions

Test temperature:
in accordance with the OECD guideline 222
The moisture content was 40 to 60% of the maximum water holding capacity. Losses are replenished as necessary with de-ionised water. The water content should not vary by more than 10 % from that at the start of the test.
Details on test conditions:
in accordance with the OECD guideline 222
Nominal and measured concentrations:
The range of test concentrations is selected to encompass those likely to cause both sub-lethal and lethal effects over a period of eight weeks.

7 2.5 0 5 0 2.5 0 7.5
14 5 0 7.5 5 5 2.5 10
Reference substance (positive control):

Results and discussion

Applicant's summary and conclusion

Executive summary:

Taking account of the results of initial CSA (see section 10) and the intrinsic properties of BDP, EPM estimation together with a confirmatory long-term toxicity test is enough to evaluate the potential risk in use of test substance. Here we choose the long-term toxicity test on earthworms as the confirmatory test.