Registration Dossier
Registration Dossier
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EC number: 425-220-8 | CAS number: 5945-33-5
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study planned
- Study period:
- Nov. 1 2011 to Feb 1 2012
Data source
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 425-220-8
- EC Name:
- -
- Cas Number:
- 5945-33-5
- Molecular formula:
- C39H34O8P2
- IUPAC Name:
- (1-methylethylidene)di-4,1-phenylenetetraphenyl diphosphate
- Details on test material:
- Same with the substance notified in the section 1 of this dossier
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Firstly, 1 g BDP is weighed by electric balance and then dissolved with dimethylformamide in 50 ml flask to get 20 g/l liquor. After that, 400,160,64 and 25.6 µL liquor is transferred into the 1 L flasks,repectively,and dissolved with M4 medium to get the 8,3.2,1.28 and 0.512mg/l test solution.
25.6,10.24 and 4.096 ml solution taking from the 8 mg/l test solution is transferred into the 1L flasks,repectively,and dissolved with M4 medium to get the 0.205,0.082 and 0.033 test solution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Obey with the OECD guideline 211.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- The fully defined medium M4 media(see SOP EX015) is used in the laboratory.
- Test temperature:
- 20±1°C
- pH:
- 7.8±0.2
- Dissolved oxygen:
- The dissolved oxygen concentration should be above 3 mg/l. In order to meet criteria, the test vessels should be aerated for 6 hours before the culture.
- Salinity:
- The fully defined medium M4 media (see SOP EX015) is used in the laboratory.
- Nominal and measured concentrations:
- Nominal concertrations 5mg/l.
- Details on test conditions:
- Obey with the OECD guideline 211.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Applicant's summary and conclusion
- Executive summary:
According to Directive 67/548/EECandCommission Regulation (EC) No: 790/2009, classification for BDPasR53and asaquatic chronic category 4, respectively,isnot assuredbased on the available data on aquatic environment, because of the absence of chronic toxicity evidences. If the chronic toxicity NOECs > water solubility or > 1 mg/L，the classification of BDP as R53 oraquatic chronic category 4will be revised and abolished. Therefore, the long-term toxicity study on Daphnia needs to be proposed.
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