Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study, monitoring.

Data source

Reference
Reference Type:
publication
Title:
Influence of genetic and environmental factors on the tolerance of daphnia magna straus to essential and non-essential metals
Author:
Barata C et al.
Year:
1998
Bibliographic source:
Aquatic toxicol 42: 115-137

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1981
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Analytical purity: reagent grade

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Clone C, Clone F
- Age at study initiation (mean and range, SD): < 24h
- Method of breeding: For each genotype, two 2 L bulk cultures of 15 animais were maintained at the
desired water hardness with the addition of a standard organic extract (Baird et al. 1989). Animais were fed daily with Chlorella vulgaris Beijerinck (1 x 10E6 cells ml ). The culture medium was changed twice weekly and neonates removed within 24 h of release.
- Feeding during test: no

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
Moderate-hard water: 90.7 mg/L CaCO3
Hard water: 179 mg/L CaCO3
Test temperature:
20±0.5°C
pH:
Moderate-hard water: 7.73±0.006
Hard water: 8.07±0.007
Dissolved oxygen:
near 100% of saturation
Details on test conditions:
TEST SYSTEM
- Test vessel:
- fill volume: 120 ml
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic inorganic medium (APHA 1995)
- Total organic carbon: 0.41-0.48 mg/L
*for more details see study


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality observed by immobility


TEST CONCENTRATIONS
- Test concentration: 8-15

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.006 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Remarks:
CH2Cu2O5
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.003 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Cu
Basis for effect:
mortality

Any other information on results incl. tables

In this study the toxicity of copper to two different Daphnia magna clones (which are generally used in toxicity testing) were examined. Clone F was significantly more sensitive (LC50 48h = 3.2 µg/L Cu moderate hard water; LC50 48h = 23.1 µg/L Cu hard water) than clone C (LC50 48h = 15.4 µg/L Cu moderate hard water; LC50 48h = 119 µg/L Cu hard water).

LC50 48h = 3.2 µg/L Cu = 0.012 mg/L CuSO4*5H2O

Applicant's summary and conclusion