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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions. Endpoint EC50 is not in accordance with today´s guideline demands.

Data source

Reference
Reference Type:
publication
Title:
Evaluation of the OECD activated sludge, respiration inhibition test
Author:
Klecka GM, Landi LP
Year:
1985
Bibliographic source:
Chemosphere 14(9): 1239-1251

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Version / remarks:
1981
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): CuSO4*5H2O
- Analytical purity: reagent grade

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Fresh stock solution has been prepared (< 2 days); concentration: 0.5 to 5.0 g/L; pH value after correction: 7.5

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Method of cultivation: Activated sludge from a local municipal wastewater treatment plant
- Preparation of inoculum for exposure: The inoculum was washed and aerated during the night. 200 ml were added to a total volume of 500 ml.
- Initial biomass concentration: 800 mg/L dw

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
21°C
pH:
7.4-8.0 during test, 7.5 after correction at the beginning
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, BOD-flask, liquid volume: 500 ml
- Aeration: sparged with air

TEST MEDIUM / WATER PARAMETERS
- Culture medium: OECD test medium

OTHER TEST CONDITIONS
- Adjustment of pH: no pH adjustments during test

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
O2: on line measurement, 5 min
Reference substance (positive control):
yes
Remarks:
3.5-dichlorophenol (CAS 591-35-5)

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
15.1 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Cu
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
26.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
CH2Cu2O5
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Reported statistics and error estimates:
CI = 38.2-53.7 mg/l

Applicant's summary and conclusion