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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Monst sensititive study as given in U.S. EPA (2007). Test concentrations were measured. Semistatic test.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Effect of food type on the acute and chronic toxicity of copper to Daphnia magna
Author:
Winner RW et al.
Year:
1985
Bibliographic source:
Freshwater Biology 7: 343-349
Reference Type:
secondary source
Title:
Aquatic life ambient freshwater quality criteria – Copper, 2007 Revision
Author:
U.S. EPA
Year:
2007
Bibliographic source:
EPA-822-R-07-001, Feb 2007

Materials and methods

Principles of method if other than guideline:
Effects of water hardness and humic acid on the chronic toxicity of Daphnia was tested, 42 days, semistatic
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Analytical purity: reagent grade

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia pulex

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
42 d

Results and discussion

Effect concentrationsopen allclose all
Duration:
42 d
Dose descriptor:
NOEC
Effect conc.:
0.005 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Cu
Basis for effect:
mortality
Remarks on result:
other: 58 mg/L CaCO3
Duration:
42 d
Dose descriptor:
NOEC
Effect conc.:
0.007 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Cu
Basis for effect:
mortality
Remarks on result:
other: 115 mg/L CaCO3
Duration:
42 d
Dose descriptor:
NOEC
Effect conc.:
0.012 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Cu
Basis for effect:
mortality
Remarks on result:
other: 230 mg/L CaCO3
Duration:
42 d
Dose descriptor:
NOEC
Effect conc.:
0.009
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Remarks:
CH2Cu2O5
Basis for effect:
mortality
Remarks on result:
other: 58 mg/L CaCO3

Any other information on results incl. tables

Contrary to the expectation that sublethal endpoints are more sensitive indicators of chronic toxicity, reproduction was not a sensitive indicator of copper stress in this species. Water hardness also had little effect on the chronic toxicity of copper (similar to D. magna trends), but humic acid significantly reduced chronic toxicity of copper when added to the varying water types. The survival chronic values based on the NOAEC and LOAEC values for the three low to no humic acid studies were 4.90, 7.07, and 12.25 μg/L total copper at hardnesses of 57.5, 115, and 230 (0.15 mg/L HA) μg/L as CaCO3, respectively. The EC20 values calculated for the low and high hardness studies using nonlinear regression techniques were 2.83 and 9.16 μg/L at hardness values of 57.5 and 230 (0.15 mg/L HA) μg/L as CaCO3, respectively.

Applicant's summary and conclusion