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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study, most sensitive NOEC in EHC 200 for algae toxicity.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Biotests using unicellular algae and ciliates for predicting long-term effects of toxicants
Author:
Schaefer H et al.
Year:
1994
Bibliographic source:
Ecotoxicol Environ Saf 27: 64-81
Reference Type:
secondary source
Title:
Environmental Health Criteria (EHC) 200, Copper
Author:
WHO
Year:
1998

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
1984
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Analytical purity: p.a.

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Chlamydomonas reinhardtii
Details on test organisms:
TEST ORGANISM
- Common name: Chlamydomonas reinhardtii
- Strain: 11-3Qa
- Source (laboratory, culture collection): SAG

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
20°C
pH:
6.2
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glas, erlenmayer flasks, yes
- Aeration: no
- Initial cells density: 2*10E5 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes, SAG medium 12

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24h
- Light intensity and quality: 8000 lux, cool white fluorescent lamps


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter

Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.14 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Remarks:
CH2Cu2O5
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.009 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Remarks:
CH2Cu2O5
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.079 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Cu
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.005 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Cu
Basis for effect:
growth rate

Any other information on results incl. tables

In addition a flow through test was performed, resulting in: NOEC (96h) = 0.026 mg/L Cu and EC50 (96h) = 0.047 mg/L Cu.

Applicant's summary and conclusion