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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, no analytical dose verification. Most sensitive LC50 for the acute toxicity to fish as given in EHC 200 (1998). Soft water was used.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Comparative toxicity of inorganic contaminants released by placer mining to early life stages of salmonids.
Author:
Buhl KJ, Hamilton SJ
Year:
1990
Bibliographic source:
Ecotox Environm Safety 20: 325-342
Reference Type:
secondary source
Title:
Environmental Health Criteria (EHC) 200, Copper
Author:
WHO
Year:
1998

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: APHA (1988). E-729-88.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Analytical purity: "reagent grade or the highest purity available"

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Thymallus arcticus; Oncorhynchus mykiss
Details on test organisms:
TEST ORGANISM
- Common name: Arctic grayling
- Source: Clear state hatchery, Clear, Alaska
- Weight at study initiation (mean and range, SD): 0.34 g
- Feeding during test: no


ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): yes

TEST ORGANISM
- Common name: Rainbow trout
- Source: Ennis National Fish Hatchery. Ennis, 1986 Montana
- Weight at study initiation (mean and range, SD): 0.6 g
- Feeding during test: no


ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): yes


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
41.3 mg/L CaCO3 total hardness
Test temperature:
12±1°C
pH:
7.1-8.0
Nominal and measured concentrations:
6-10 test concentrations and control were tested
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.003 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Cu
Basis for effect:
mortality
Remarks on result:
other: Arctic grayling
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.014 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Cu
Basis for effect:
mortality
Remarks on result:
other: Rainbow trout
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.004 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
CH2Cu2O5
Basis for effect:
mortality
Remarks on result:
other: Arctic grayling
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.024 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
CH2Cu2O5
Basis for effect:
mortality
Remarks on result:
other: Rainbow trout

Applicant's summary and conclusion