Ditetradecyl peroxydicarbonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

adopted 4 April 1984

Deviations:

yes

Remarks:

other reference substance: 2,4,5-trichlorophenol

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Version / remarks:

1988

Deviations:

yes

Remarks:

other reference substance: 2,4,5-trichlorophenol

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was absorbed onto silica gel using a stock solution of 10 g/litre in dichloromethane.
- Controls: yes
- Chemical name of vehicle: dichloromethane
- Concentration of vehicle in test medium: 10 g/L in the stock solution

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Laboratory culture: no (Source: RZWI Nieuwgraaf in Duiven)
- Preparation of inoculum for exposure: The activated sludge plant predominantly treats domestic wastewater. Prior to use, the activated sludge was diluted with tap water (1.5 x) and hmogenized for two minutes in a lab blender 400.
- Initial biomass concentration: The activated sludge concentration in the incubation vessels was 0.5 g dry weight/L.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Test temperature:

20 °C

pH:

7.5 to 7.9

Dissolved oxygen:

0.36 - 0.37 mg O2/L min

Nominal and measured concentrations:

- nominal concentration: 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: The volume of the vessel was 10 mL.
- Type: closed (by the electrode to measure the oxygen depletion)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Intervals of water quality measurement: The pH of the reaction mixture was measured before the respiration measurement.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The respiration rates of the activated sludge were measured in a Biological Oxygen Monitor YSI5300.

Reference substance (positive control):

yes

Remarks:

2,4,5-trichlorophenol

Key result

Duration:

30 min

Dose descriptor:

NOEC

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

No inhibition of the respiration rate occured at 1000 mg/L of the test item absorbed onto silica gel. This result shows that the test item, at the obtained distribution, is not toxic for activated sludge.

Results with reference substance (positive control):

The respiration rate of 86 % was occured at 40 mg/L of 2, 4, 5-trichlorophenol.

Validity criteria fulfilled:

yes

Conclusions:

No inhibition of the respiration rate occured at 1000 mg/L of ditetradecyl peroxydicarbonate absorbed onto silica gel. This result shows that the test item, at the obtained distribution, is not toxic for activated sludge.

Executive summary:

Ditetradecyl peroxydicarbonate was tested in an activated sludge respiration inhibition test according EU method C.11 and OECD guideline No. 209. The test substance was not soluble in water and therefore the activated sludge was exposed to ditetradecyl peroxydicarbonate adsorbed onto silica gel at a concentration of 1 g/L. By this exposure no effect was recorded indicating that the obtained distribution of the test substance in the water system did not inhibit the activated sludge.

No inhibition of the respiration rate occured at 1000 mg/L of ditetradecyl peroxydicarbonate absorbed onto silica gel. This result shows that the test item, at the obtained distribution, is not toxic for activated sludge.

Description of key information

No inhibition of the respiration rate occurred at 1000 mg/L of ditetradecyl peroxydicarbonate absorbed onto silica gel. This result shows that the test item, at the obtained distribution, is not toxic for activated sludge.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

1 000 mg/L

Additional information

Ditetradecyl peroxydicarbonate was tested in an activated sludge respiration inhibition test according EU method C.11 and OECD guideline No. 209. The test substance was not soluble in water and therefore the activated sludge was exposed to ditetradecyl peroxydicarbonate adsorbed onto silica gel at a concentration of 1 g/L. By this exposure no effect was recorded indicating that the obtained distribution of the test substance in the water system did not inhibit the activated sludge. No inhibition of the respiration rate occured at 1000 mg/L of ditetradecyl peroxydicarbonate absorbed onto silica gel. This result shows that the test item, at the obtained distribution, is not toxic for activated sludge.