Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997-04-03 till 1997-05-15
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions.
according to guideline
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
GLP compliance:
Analytical monitoring:
Details on sampling:
- Concentration of 10000 mg/L and control were measured at 24 and 48 hours.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Daphnia mangna STRAUS parthenogenetic females, strain of the Federal Health Office Berlin
- Starting strain: Clone breeding the Federal Health Office Berlin
- Test animals: laboratory breeding of Bayer AG Leverkusen
- Age of test aminmals: 0 - 24 hours
Test type:
Water media type:
Total exposure duration:
48 h
13.8 °dH
Test temperature:
20.4 - 20.6 °C after 48 hours
7.8 - 7.9 after 48 hours
Dissolved oxygen:
9.9 - 10.0 mg O2/L after 48 hours
Nominal and measured concentrations:
10000 mg/L (nominal) plus control
Details on test conditions:
- Test method: cylindrical test vessels, diameter 4.0 cm, height 6.5 cm; 20 mL test medium; not aerated
- Application method: direct weighing
- 10 neonates per vessel, 2 replicates per concentration/control
- The weighed substance in water was used to prepare single test approach 1 hour in an ultrasonic bath treated and then for 24 hours stirred on a magnetic stirrer.
- Undissolved percentage substance were determined by a pleated filter (pore size 7-12 microns) filtered off.
- Criteria of effects: item-induced alteration of the normal mobility and the loss of locomotory actions of the neonates, observed at 24 and 48 hours.
Reference substance (positive control):
48 h
Dose descriptor:
Effect conc.:
>= 10 000 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:

No inmobilisation and abnormalities (abnormal coloration or abnormal swimming behavior) were observed at 24 and 48 hours and any concentrations.

Validity criteria fulfilled:
not specified
Daphnia magna was exposed under static conditions for 48 hours to one concentrion (10000 mg/L) of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one. No toxic effect was observed.
Executive summary:

The acute toxicity to invertebrates of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one was determined according to the EU method C.2 "Acute Toxicity for Daphnia" (1992). Daphnia magna was exposed to the test solution of one nominal concentration of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one (10000 mg/L) and blank control solution for a period of 48 hours under static conditions. No toxic effect and abnormal behavior were observed.

Description of key information

Daphnia magna were exposed under static conditions for 48 hours to 10000 mg/L of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one. No toxic effect was observed. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
10 000 mg/L

Additional information

The key value should read as EC0 ≥ 10000 mg/L as no effect was reported up to the highest test concentration.