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EC number: 701-184-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 9 July 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: US EPA 1979
- Deviations:
- not applicable
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sampling method: concentrations were determined on days 0, 2, 4, 7, 9, and 11.
- Sample storage conditions before analysis: not reported - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: direct weights of the test substance, corrected for active acid, were added directly to the test vessels. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: fishery called Creek hatchery in Lewiston, Montana, USA.
- Length at study initiation (length definition, mean, range and SD): initial mean standard length 34.3 mm
- Weight at study initiation (mean and range, SD): initial mean weight of 0.57g
- Method of breeding: not reported
- Feeding during test
- Food type: commercial fish food Rangen's
- Amount: 3% body weight
- Frequency: daily
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): similar conditions to test: holding tanks on 16 h daylight period.
- Type and amount of food: commercial fish food Rangen's, 3% body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not reported - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Hardness:
- 255 ppm as CacO3 in the dilution water
- pH:
- control: 8.1-8.6
low concentration: 7.6-8.4 (new solution) 8.3-8.6 (old solution)
high concentration: 5.8-6.6 (new solution) 8.4-8.5 (old solution)
The pH increased during the Friday to Monday water renewal periods. It is thought by the authors of the report that this is due to the aeration put in place on day 6. - Dissolved oxygen:
- control: 8.2-10 mg/l
low concentration: 8.8-9.5 (new solution), 8.5-8.9 (old solution)
high concentration: 9.2-10.5 (new solution), 9.0-9.2 (old solution) - Nominal and measured concentrations:
- Nominal concentrations: 0, 75, 140, 240, 420, 750 mg active acid/l
Measured concentrations: 0, 74, 137, 232, 408 and 703 mg active acid/l - Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 40 L glass aquaria, filled to 30 L of test solution
- Aeration: aeration took place on days 6, 8, 10, 12, 13 and 14 by gently bubbling compressed air, when DO levels were approaching 40%.
- Renewal rate of test solution (frequency/flow rate): 72%, i.e. 22.5 L, were replaced on each Monday, Wednesday and Friday
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water
- Metals: all <0.01 ppm, except Iron 0.012 ppm and Nickel 0.0157 ppm
- Pesticides:
- Alkalinity: 368 ppm as CacO3
- Conductivity: 50 uhmos/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: water quality parameters were measured on each renewal date (Mon, Wed, Fri) in low, medium and high concentrations.
OTHER TEST CONDITIONS
- Adjustment of pH: none reported
- Photoperiod: 16 h daylight
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : behaviour and mortality were observed daily.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7 to 1.9
- Justification for using less concentrations than requested by guideline: n/a
- Range finding study: none reported - Reference substance (positive control):
- yes
- Remarks:
- Antimycin A
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 440 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- active acid
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: (350-560)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 440 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- active acid
- Basis for effect:
- mortality (fish)
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 74 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- active acid
- Basis for effect:
- behaviour
- Remarks:
- loss of equilibrium
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 232 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Remarks:
- active acid
- Basis for effect:
- mortality (fish)
- Details on results:
- - Observations on body length and weight: weight measurements of surviving fish at the end of the study (in percentages of the control group mean weight): 74 mg/l 90%; 137 mg/l 90%; 232 mg/l 76% and 408 mg/l 74%.
- Other biological observations: although only 5% mortality was observed at 232 mg/l, all test fish at this concentrations exhibited loss of equilibrium within 30 minutes after beginning the test. All surviving test fish at this concentrations recovered from the apparent initial toxicant shock, and the same behavioural response was noted immediately following each solution change at 232 and 408 mg/l. The loss of equilibrium following solution changes was thought to be due to a pH effect, as initial pH levels decreased with increasing toxicant concentration.
- Mortality of control: <5% - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Reported statistics and error estimates:
- Litchfiled and Wilcoxon methods
- Sublethal observations / clinical signs:
Table 1. Summary of mortalities during the 14 day exposure.
Nominal concentration (mg/l) Days of exposure 1 3 14 Control 0 0 0 74 0 0 0 137 0 0 0 232 5 5 5 408 40 40 40 703 95 100 100 Table 2. Mean measured total phosphorous and Dequest 2051 concentrations.
Nominal concentration (mg/l) Mean P Dequest 2051 Control -- -- 75 19.3 74 140 35.5 137 240 60.2 232 420 106 408 750 183 703 - Validity criteria fulfilled:
- yes
- Remarks:
- however, the pH and DO were low at the high concentration
- Conclusions:
- A 72h and 14d LC50 value of 440 mg active acid/L have been determined for the effects of the test substance on the mortality of the freshwater fish Oncorhynchus mykiss (reported as Salmo gairdneri) based on arithmetic mean measured concentrations. A NOEC value of 232 mg active acid/L based on mortalities has also been reported in the same study.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: US EPA 1979
- Deviations:
- not applicable
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- no range finding test and no analytical monitoring
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: an appropriate amount of test substance was added to each vessel and dispersed
- Controls: sea water only - Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: sheepshead minnow
- Source: stocked ponds at Bionomics Marine Research Laboratory
- Length at study initiation (length definition, mean, range and SD): 15-20 mm
- Method of breeding: not reported
- Feeding during test: not fed
ACCLIMATION
- Acclimation period: min. 7 days
- Acclimation conditions (same as test or not): not reported
- Type and amount of food: not reported
- Health during acclimation (any mortality observed): <1% mortality - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 20 ± 1⁰C
- pH:
- control: 8.3 to 8.2; exposure concentrations: 6.1 to 7.1
- Dissolved oxygen:
- ≥60%
- Salinity:
- 25‰
- Nominal and measured concentrations:
- Nominal concentrations: Control, 1000, 1800, 3200, 5600 and 10 000 µl/l (ppm)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: jars
- Type (delete if not applicable): open
- Material, size, fill volume: glass, 19 L which contained 15L of seawater
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural, filtered (5 micrometers) sea water
- Intervals of water quality measurement: 24 hours
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: not reported
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortalities were recorded every 24 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 to 2
- Range finding study: not reported to have been conducted - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 3 200 - < 5 600 other: µl/l (ppm)
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 820 - < 1 430 other: µl/l (ppm)
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- active acid
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Reported statistics and error estimates:
- LC50s were calculated by linear regression
- Sublethal observations / clinical signs:
Table 1. Summary of findings.
Nominal concentration (u/l; ppm) Mortality (%) 24 h 48 h 96 h Control 0 0 0 1000 0 0 0 1800 0 0 0 3200 0 0 0 5600 100 100 100 10 000 100 100 100 Result expressed as nominal concentration. Properties of the test substance and evidence from other studies (where concentrations were
measured) indicate that nominal and measured concentrations are likely to be in good agreement.
A 96 h LC50 value between 3200 and 5600 µl/l (ppm) of test substance has been determined. This is equivalent to 820-2430 µl/l (ppm) in terms of HMDTMP active acid. The specific gravity of the test substance is 1.23 therefore the results in terms of mg/l are:
HMDTMP-xK = 96 h LC50 >3900 and <6900 mg/l, where the middle point is 5400 mg/l
HMDMTP acid = 96 h LC50 >1000 and >3000 mg/l, where the middle point is 2000 mg/l
- Validity criteria fulfilled:
- yes
- Conclusions:
- a 96 h LC50 value of >3900 <6900 mg/l of test substance, corresponding to >1000 and <3000 mg/l active acid, has been determined for the effects of the test substance on the mortality of the marine fish species Cyprinodon variegatus based on nominal concentrations.
Referenceopen allclose all
Description of key information
14 d LC50 440 mg active acid/L, O. mykiss, reliability 2 (ABC 1979, read-across from HMDTMP acid)
96 h LC50 >1000 and <3000 mg active acid/l, C. variegatus, reliability 2 (EG&G 1976)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 440 mg/L
Marine water fish
Marine water fish
- Effect concentration:
- 2 000 mg/L
Additional information
72-hour and 14-day LC50 values of 440 mg active acid/L have been determined for the effects of HMDTMP-H on the mortality of the freshwater fish Oncorhynchus mykiss (reported as Salmo gairdneri) based on arithmetic mean measured concentrations. A NOEC value of 232 mg active acid/L based on mortalities has also been reported in the same study (ABC 1979).
A 96 hour LC50 value between >3900 and <6900 mg/L of test substance, corresponding to >1000 and <3000 mg/L active acid, has been determined for the effects of the registered substance, HMDMTP(4 -7K), on the mortality of the marine fish species Cyprinodon variegatus based on nominal concentrations (EG&G 1976). The mid point of the range is 2000 mg active acid/L, which is used as the key marine water value and the 14 -day LC50 value of 440 mg active acid/L has been selected as the key freshwater value.
A supporting study also indicates low short-term toxicity of the substance to fish, where a 96 hour LC50 value of >2400 mg active acid/L with the freshwater catfish Ictalurus punctatus (Monsanto 1976) has been reported.
The acid, sodium and potassium salts in the HMDTMP category are freely soluble in water. The HMDTMP anion can be considered fully dissociated from its sodium or potassium cations when in dilute solution. Under any given conditions, the degree of ionisation of the HMDTMP species is determined by the pH of the solution. At a specific pH, the degree of ionisation is the same regardless of whether the starting material was HMDTMP-H, HMDTMP.4Na, HMDTMP.7K or another salt of HMDTMP.
Therefore, when a salt of HMDTMP is introduced into test media or the environment, the following is present (separately):
1. HMDTMP is present as HMDTMP-H or one of its ionised forms. The degree of ionisation depends upon the pH of the media and not whether HMDTMP (4-7K) salt, HMDTMP (4-7Na) salt, HMDTMP-H (acid form), or another salt was used for dosing.
2. Disassociated potassium or sodium cations. The amount of potassium or sodium present depends on which salt was added.
3. It should also be noted that divalent and trivalent cations would preferentially replace the sodium or potassium ions. These would include calcium (Ca2+), magnesium (Mg2+) and iron (Fe3+). These cations are more strongly bound by HMDTMP than potassium and sodium. This could result in HMDTMP-dication (e.g. HMDTMP-Ca, HMDTMP-Mg) and HMDTMP-trication (e.g. HMDTMP-Fe) complexes being present in solution.
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