4,4'-methylenebis[2,6-diethylaniline]

Endpoint summary

Description of key information

Short-term toxicity to fish

The purpose of this study was to evaluate the acute toxicity of the test item on Zebrafish (Brachydanio rerio). The study was perfored according to EU testing method C.1 and OECD no. 203 as semi-static test. The test item had significant toxic effects on fish. The 96-h LC50 value was determined to be 1.32 mg/L. The highest test concentration causing no mortality within the test period (96-h LC0) was determined to be 0.87 mg/L and the lowest test concentration causing 100 % mortality within the test period (96-h LC100) was determined to be higher than 1.36 mg/L. The results are based on the measured concentrations.

Short-term toxicity to aquatic invertebrates

The acute toxicity of the test item was assessed on Daphnia magna in a 48 -hour Acute Immobilisation Test. The study was performed according to EU testing method C.2 and OECD no. 202. Since there was no immobilisation or any sub-lethal effects observed up to the saturation concentration (1.69 mg/L measured) and the test item was stable at least at saturation for the duration of the test it can be stated that the test item has no toxic effects on Daphnia magna up to at least the solubility limit of the test item in the test medium (1.69 mg/L measured).

Toxicity to aquatic algae and cyanobacteria

The effect of the test item was assessed on algal growth using the unicellular green alga Pseudokirchneriella subcapitata (Selenastrum capricornutum), over an exposure period of 72 hours. The test was perfomed according to EC-method C.3 and OECD no. 201. The No Observed Effect Concentration (NOEC) was determined to be 0.19 mg/L. The Lowest Observed Effect Concentration (LOEC) was determined to be 0.41 mg/L. In conclusion, the 72-h EC50 value based on growth rate and yield was determined to be higher than the saturation concentration (1.65 mg/L measured).

Long-term toxicity to fish

The test item had significant toxic effects on fish in an acute study. The 96 -h LC50 value was determined to be 1.32 mg/L. The highest test concentration causing no mortality within the test period (96 -h LC0) was determined to be 0.87 mg/L. Fish was determined to be the most sensitive aquatic species to the test item, therefore, it's planned to conduct a long-term study on fish (juvenile growth test)..

Long-term toxicity to aquatic invertebrates

The long-term toxicity to aquatic invertebrates was waived. The substance was found to be non-toxic at the solubility limit in the acute daphnia toxicity study; furthermore it's planned to conduct a long-term toxicity study on fish, the most sensitive species, based on the results of the acute test.

Toxicity to microorganisms

The purpose of the 3-hour test was to evaluate the influence of the test item on the activity of the activated sludge by measuring the respiration rate under defined conditions. Based on measured oxygen consumption values and calculated specific respiration rates it can be stated that the 3-hour EC10 and EC50 values of the test item are greater than 1000 mg/L. The NOEC was determined to be 1000 mg/L.

Additional information

Source: GLP-reports