Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-340-2 | CAS number: 119-64-2
Cited from SIAR to SIAM 19 (Berlin, 19-22 October 2004)
In vitro Studies
In an Ames test performed according to the original publication by B. Ames (1975) with Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98, and TA 100, test substance concentrations of 10; 50; 100; 250; 500; 1000; 5000 μg/plate were employed in the presence and absence of Aroclor induced rat liver S9 mix. Cytotoxicity was observed at > 50 μg/plate (-S9) and > 500 μg/plate (+S9), respectively. At non-toxic test substance concentrations, a significant increase in mutant frequency was not observed (Hüls AG, 1988). A negative result in the Ames test (+/- Aroclor 1254 induced rat liver S9 mix) was also obtained by Florin et al. (1980) using the same strains except TA 1538 and doses of 0.03; 0.3; 3; 30 μmol/plate (ca. 4; 40; 400; 4,000 μg/plate). These authors observed cytotoxicity at >= 3 μmol/plate (ca. 400 μg/plate). The chemical was also tested negative in an Ames test performed within the frame of the U.S. National Toxicology Program (NTP, 2004)
In a mouse lymphoma test, a negative result was obtained in the absence of a metabolic activation system (dose range 35-47.5 μg/ml), while with the addition of S-9 mix the test was equivocal (dose range 10-20 μg/ml): The only cultures exhibiting a significant increase in mutant frequency had less than 10 % total growth (National Cancer Institute / Microbiological Associates, 1992).
In vivo Studies
In a micronucleus assay in NMRI mice according to OECD TG 474 (1983) performed by Hüls AG
(1993), five mice per sex and test duration received one single application with 2,000 mg 1,2,3,4-
tetrahydronaphthalene/kg bw in corn oil (10 ml/kg bw) by gavage. The selected dose was the
maximum dose without mortalities within 48 hours. Sampling times were 24 and 48 hours after test
substance administration. 1,2,3,4-Tetrahydronaphthalene treatment did not result in an increase in
the frequency of micronucleated polychromatic erythrocytes (PCE), but it significantly reduced the
PCE/NCE ratio in male and female animals at both sampling times. This clearly indicates that the
target organ (the bone marrow) had been reached in this test.
1,2,3,4-Tetrahydronaphthalene did not induce micronuclei in peripheral erythrocytes taken from
B6C3F1 mice (10 per dose and sex) which were exposed for 13 weeks on 6 h/day, 5 days/week to
concentrations of 7.5; 15; 30; 60; 120 ppm = 41.2; 82.4; 165; 330; 660 mg/m3 (NTP, 1997 a;
Studies in Humans
There were no studies on humans available.
1,2,3,4-Tetrahydronaphthalene was not genotoxic in bacterial systems in vitro (Ames test). In a Mouse Lymphoma test, results were negative and equivocal, without and with metabolic activation, respectively. In vivo, no mutagenic activity was detectable in two micronucleus assays on mice according to OECD TG 474 using the oral and inhalation routes of administration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again