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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from secondary literature

Data source

Reference
Reference Type:
secondary source
Title:
Acute Vapor inhalation Toxicity Study for CAS no. 75-87-6
Author:
NTRL report
Year:
1992
Bibliographic source:
NTRL report ,OTS0555527, 1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
other: As mention below
Principles of method if other than guideline:
Acute inhalation toxicity study of test chemical in albino rats
GLP compliance:
not specified
Test type:
other: not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Trichloroacetaldehyde
EC Number:
200-911-5
EC Name:
Trichloroacetaldehyde
Cas Number:
75-87-6
Molecular formula:
C2HCl3O
IUPAC Name:
trichloroacetaldehyde
Details on test material:
Name of test material (as cited in study report):2, 2, 2-trichloroacetaldehyde
- Molecular formula :C2HCI3O
- Molecular weight :147.387 g/mole
- Substance type:organic
- Physical state:Liquid
Purity:No data available
- Impurities (identity and concentrations):No data available

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
Details on test animal
TEST ANIMALS
- Source: No data available
- Age at study initiation: Specific age was not mention young rats were taken
- Weight at study initiation: 144 grams
- Fasting period before study: No data available
- Housing: in a stock cages
- Diet (e.g. ad libitum): standard laboratory diet (Purina Rat Chow)ad libitum
- Water (e.g. ad libitum): water ad libitum
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25°C
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available

IN-LIFE DATES: From: To: No data available

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
clean air
Remark on MMAD/GSD:
No data available
Details on inhalation exposure:
No data available
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Remarks on duration:
total : 240min
Concentrations:
0.24,0.36,0.38,0.71,6.32 mg/l
No. of animals per sex per dose:
Total :50
0.24mg/l :10
0.36mg/l:10
0.38mg/l:10
0.71mg/l:10
6.32mg/l:10
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Weighing: Before and after inhalation
- Necropsy of survivors performed: yes
- Other examinations performed:
clinical signs: Yes
body weight: Yes, Before and after 14 days of observation
organ weights: yes
histopathology: No data available
other:
Statistics:
Litchfield and Wilcoxon

Results and discussion

Preliminary study:
No data available
Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
0.44 mg/L air
Based on:
test mat.
95% CL:
> 0.38 - < 0.5
Exp. duration:
240 min
Remarks on result:
other: mortality was observed
Mortality:
Mortality was observed at dose 0.36 and 0.38 at four to five days of exposure and at dose 0.71 and 6.32 death was observed at one to three days.
Clinical signs:
other: Sneezing was noted approximately one-half hour after the start of the inhalation period. Hypoactivity, ptosis, and dyspnea occurred at about one hour, with gasping and prostration at two to three hours.
Body weight:
At dose 0.24,0.36 and 0.38 body weight was observed as 70, 39 and 58 grams all are within the normal limits
Gross pathology:
Moderate to severe diffuse red discoloration of the lungs were seen. Three rats from Group 2 did not reveal any gross pathologic alteration
Other findings:
No data available

Any other information on results incl. tables

Acute Vapour Inhalation Toxicity study- Albino Rats

                              Mortality Data

Group

Average. Nominal

Vapour Concentration

Number of Animals tested

Observed precent death

Expected percent death

I

0.24

10

0

0.01

II

0.36

10

10

12.10

III

0.38

10

20

20.00

VI

0.71

10

100

99.86

V

6.32

10

100

100

Acute Vapour Inhalation LC50 = 0.44mg,/L air (four-hour exposure)

LimitsofLC50 at the 95 %Levelofconfidence=0.38 – 0.50mg/L air

 

Applicant's summary and conclusion

Interpretation of results:
Category 1 based on GHS criteria
Conclusions:
The LC50 value was considered to be 0.44 mg/l of air (vapour) .When rats were inhaled with the given test chemical.
Executive summary:

Acute inhalation toxicity test was conducted in male rats of Sprague-Dawley strain. 5 groups of 10 rats were taken for test. Rats were exposed for 4 hrs and observed for 14 days. Body weight was measured before and after 14 days of observation. Gross pathology was conducted for the animal which died during test and after completion of observation period. At dose 0.24,0.36 and 0.38 body weight was observed as 70, 39 and 58 grams all are within the normal limits. Moderate to severe diffuse red discoloration of the lungs were seen. Mortality was observed at  dose 0.36 and 0.38 at four to five days of exposure and at dose 0.71 and 6.32 death was observed at one to three days. Therefore, the LC50  was considered to be 0.44 mg/l of air (vapour) .When rats were inhaled with the given test chemical.