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Link to relevant study record(s)

Reference
Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Data is from peer-reviewed journal
Objective of study:
metabolism
Qualifier:
according to guideline
Guideline:
other: as below
Principles of method if other than guideline:
In Vivo metabolism study of 2,3-dimethylphenol in rabbit
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): 2,3-dimethylphenol
- Molecular formula (if other than submission substance): C8H10O
- Molecular weight (if other than submission substance): 122.166 g/mole
- Substance type: Organic
- Physical state: No data available
- Impurities (identity and concentrations): No data available
Radiolabelling:
not specified
Species:
rabbit
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: No data available
- Age at study initiation:No data available
- Weight at study initiation: 2-3 kg.
- Fasting period before study:No data available
- Housing:No data available
- Diet (e.g. ad libitum): rabbit pellets, ad libitum
- Water (e.g. ad libitum):Water, ad libitum
- Acclimation period:No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C):No data available
- Humidity (%):No data available
- Air changes (per hr):No data available
- Photoperiod (hrs dark / hrs light):No data available

IN-LIFE DATES: From: To:No data available
Route of administration:
oral: gavage
Vehicle:
water
Details on exposure:
2,3-dimethylphenol were administered by stomach tube as suspensions in water.
Duration and frequency of treatment / exposure:
No data available
Dose / conc.:
1 000 mg/kg bw/day
No. of animals per sex per dose / concentration:
No data available
Control animals:
not specified
Positive control reference chemical:
No data available
Details on study design:
No data available
Details on dosing and sampling:
No data available
Statistics:
No data available
Preliminary studies:
No data available
Type:
absorption
Results:
No data available
Type:
distribution
Results:
No data available
Type:
metabolism
Results:
Metabolism taks plase by O-conjugates and give , ethereal sulphate, ester glucuronide, ether glucuronide and ether-soluble acid
Type:
excretion
Results:
excreted in urine
Details on absorption:
No data available
Details on distribution in tissues:
No data available
Details on excretion:
1% free non-acidic phenol, 13% ethereal sulphate, 1 % ester glucuronide, 50 % ether glucuronide and 69 % ether-soluble acid were recovered in urine
Metabolites identified:
yes
Details on metabolites:
The major portion of the dose (77-93 %) being excreted as O-conjugates. the values for ether glucuronide are somewhat lower than those for the ether-soluble acid. This difference probably arises from decomposition occurring during the preliminary hydrolysis which case the crystals obtained were shown by comparison is carried out in 5N-sulphuric acid.

Excretion of metabolites of xylenols in the rabbit

 

 

Excretion

2:3-Xylen-1-ol

No. Of Experiments

Range (%)

Average (%)

Free non-acidic phenol

4

0-4-1-6

1

Ethereal sulphate

3

11-14

13

Ester glucuronide

6

0-3

1

Ether glucuronide

6

37-69

50

Ether-soluble acid

6

55-86

69

Conclusions:
Interpretation of results (migrated information): low bioaccumulation potential based on study results
On the basis of observed result it is concluded that 2,3-dimethylphenol show O-conjugates metabolism and excreted in the form of ethereal sulphate, ester glucuronide, ether glucuronide and ether-soluble acid in urine. Hence, expected to have Low bio-accumulation potential based on study results. 
Executive summary:

In a in vivo metabolism study, rabbit were treated with 2,3-dimethylphenol orally by gavage at 1000 mg. It was observed that 2,3-dimethylphenol were excreted as O-conjugates by preliminary hydrolysis which case the crystals obtained were shown by comparison is carried out in 5N-sulphuric acid. 1% free non-acidic phenol, 13% ethereal sulphate, 1 % ester glucuronide, 50 % ether glucuronide and 69 % ether-soluble acid were recovered in urine.Therefore, On the basis of observed result it is concluded that 2,3-dimethylphenol show O-conjugates metabolism and excreted in the form of ethereal sulphate, ester glucuronide, ether glucuronide and ether-soluble acid in urine. Hence, expected to have Low bio-accumulation potential based on study results. 

Description of key information

In a in vivo metabolism study, rabbit were treated with 2,3-dimethylphenol orally by gavage at 1000 mg. It was observed that 2,3-dimethylphenol were excreted as O-conjugates by preliminary hydrolysis which case the crystals obtained were shown by comparison is carried out in 5N-sulphuric acid. 1% free non-acidic phenol, 13% ethereal sulphate, 1 % ester glucuronide, 50 % ether glucuronide and 69 % ether-soluble acid were recovered in urine.Therefore, On the basis of observed result it is concluded that 2,3-dimethylphenol show O-conjugates metabolism and excreted in the form of ethereal sulphate, ester glucuronide, ether glucuronide and ether-soluble acid in urine. Hence, expected to have Low bio-accumulation potential based on study results. 

Key value for chemical safety assessment

Bioaccumulation potential:
low bioaccumulation potential

Additional information

In a in vivo metabolism study, rabbit were treated with 2,3-dimethylphenol orally by gavage at 1000 mg. It was observed that 2,3-dimethylphenol were excreted as O-conjugates by preliminary hydrolysis which case the crystals obtained were shown by comparison is carried out in 5N-sulphuric acid. 1% free non-acidic phenol, 13% ethereal sulphate, 1 % ester glucuronide, 50 % ether glucuronide and 69 % ether-soluble acid were recovered in urine.Therefore, On the basis of observed result it is concluded that 2,3-dimethylphenol show O-conjugates metabolism and excreted in the form of ethereal sulphate, ester glucuronide, ether glucuronide and ether-soluble acid in urine. Hence, expected to have Low bio-accumulation potential based on study results.