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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Qualifier:
according to
Guideline:
other: refer below principle
Principles of method if other than guideline:
The toxic effect of 2, 3 Dimethylphenol in fish species Gadus morhua L (egg and larva) was observed for 96 h.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): 2,3-dimethylphenol
- Molecular formula (if other than submission substance): C8H10O
- Molecular weight (if other than submission substance): 122.16 g/mol
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
yes
Details on sampling:
No data available
Vehicle:
yes
Details on test solutions:
No data
Test organisms (species):
Gadus morrhua
Details on test organisms:
- Age at study initiation (mean and range, SD):First day of fertilization
- egg and larva of the test organism were used
Test type:
static
Water media type:
saltwater
Total exposure duration:
96 h
Post exposure observation period:
No data
Hardness:
No data
Test temperature:
5° C
pH:
No data
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
No data
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed, test beakers covered with aluminum foil
- Material, size, headspace, fill volume: Test beakers containing 100 ml filtered seawater mixed with the test substance.
- No. of organisms per vessel: 50 fish eggs, or 20 larvae.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Yes ,Test organisms were observed on 1 ,2 and 4 the day
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
13 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Multiple effect
Details on results:
- Other biological observations: The earliest pigment was visible shortly before exposure, and the treatment produced serious pigment defects. At hatching, after exposure to 10 mg/litre, body pigmentation was totally inhibited, and only the eyes had a pale colour
- Abnormal responses: Effects on development were visible as irregular and deformed embryos.
Validity criteria fulfilled:
not specified
Conclusions:
EC50 was considered to be 13 mg/l for fish species Gadus morhua L (egg and larva) based on mortality when they were exposed to 2, 3 Dimethylphenol for 96h under static condition.
Executive summary:

In a Short-term toxicity to fish study for 2, 3 Dimethylphenol in Gadus morhua L (egg and larva) .

EC50 was considered to be 13 mg/l for 2, 3 Dimethylphenol when Gadus morhua L (egg and larva) were exposed for 96h under static condition.

Mentioned effect concentration (EC50) for 96 hrs exposure period suggest that the test chemical 2, 3 Dimethylphenol was toxic to fish and thus consider for aquatic category 3 as per the CLP criteria.

Description of key information

In a Short-term toxicity to fish study ( Inger-Britt Falk-Petersen et al; Sarsia, 29 May 1985, Vol. 70, Issue 1, pages 11 – 16) for 2, 3 Dimethylphenol in Gadus morhua L (egg and larva). The EC50 was considered to be 13 mg/l for 2, 3 Dimethylphenol when Gadus morhua L (egg and larva) were exposed for 96h under static condition.

Mentioned effect concentration (EC50) for 96 hrs exposure period suggest that the test chemical2, 3 Dimethylphenol was toxic to fish and thus consider for aquatic category 3 as per the CLP criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
13 mg/L

Additional information

Four studies including experimental data and predicted data from validated model for short term fish toxicity endpoint of target chemical 2,3-dimethylphenol (Cas no. 526-75-0) were summarized as follows:

 

In the first experimental study, short-term toxicity to fish (Inger-Britt Falk-Petersen, et al. 1985) for 2, 3 Dimethylphenol in Gadus morhua L (egg and larva) was conducted. The EC50 was considered to be 13 mg/l for 2, 3 Dimethylphenol when Gadus morhua L (egg and larva) were exposed for 96h under static condition. Mentioned effect concentration (EC50) for 96 hrs exposure period suggest that the test chemical2, 3 Dimethylphenol was toxic to fish and thus consider for aquatic category 3 as per the CLP criteria.

 

Above aquatic classification supported by other evidence i.e short term toxicity study was conducted by Devillers,J., T. Meunier, and P. Chambon (1985) to determine the effects of test chemical 2,3 Dimethylphenol on Danio rerio for 24 hours under static condition. The study indicates LC50 was considered to be 10-35mg/l for Danio rerio based on mortality effect. Thus based on the lethal concentration it is concluded that the test substance 2, 3 Dimethylphenol was toxic to fish for acute exposure period.

 

Another experimental study from same data source i.e Devillers,J., et al; Tech. Sci. Munic.80:329-334, (1985) suggest Short term toxicity study was conducted to test the effects 2, 3 Dimethylphenol on Danio rerio for 6 hours under static condition. The LC50 at 2 hours was considered to be 35-100 mg/L for Danio rerio based on mortality effects.

 

Last data from Danish QSAR database using three different models i.e, Battery, Leadscope and SciQSAR indicate estimated 96 hrs LC50 value of test substance 2,3-Dimethylphenol on Fathead minnow was determined to be 20.21 mg/l.

Thus above all studies indicate that the target chemical 2, 3 Dimethylphenol (Cas no.526-75-0) is likely to be toxic to fish and classified in Chronic category 3 as per the CLP criteria for aquatic classification.