Methanol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Remarks:

intraperitoneal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Toxicological Research of Methanol as a fuel for Power Station. Summary Report on Tests with Monkeys, Rats and Mice.

Author:

New Energy Development Organization

Year:

1987

Bibliographic source:

New Energy Development Organization, Tokyo

Materials and methods

Principles of method if other than guideline:

Determination of the lethal dose after intraperitoneal application of the test substance to monkeys in the context of a metabolism study.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

Molecular formula:

CH4O

IUPAC Name:

methanol

Test animals

Species:

monkey

Strain:

Macaca fascicularis

Sex:

male

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

0, 25, 125, 600, 3000 mg/kg

No. of animals per sex per dose:

1 (a total of 5 animals was repeatedly used)

Control animals:

yes

Details on study design:

- Duration of observation period following administration: at least 2 days
- Frequency of observations and weighing: examination of clinical chemical parameters after 1, 3, 10, 24, 30, 48 hours
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, histopathology, other: clinical chemistry

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LDLo

Effect level:

3 000 mg/kg bw

Mortality:

During another part of the study 3 animals died after injection of 3000 mg/kg bw within 24 to 48 hours.

Clinical signs:

No abnormalitites were seen up to 600 mg/kg bw. All monkeys exposed to 3000 mg/kg fell into a coma immediately after administration and remained recumbent for 3 h to 2 d. Additionally, anorexia was observed.

Gross pathology:

No lesions in the eye, but slight effects in the liver.

Other findings:

- Histopathology: No lesions in the optic nerve or central nervous system
- Potential target organs: Liver
- Other observations: The highest dose level led to increased formate level, decreased pH in blood as well as delayed excretion of methanol and secondary products