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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Remarks:
intraperitoneal
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Toxicological Research of Methanol as a fuel for Power Station. Summary Report on Tests with Monkeys, Rats and Mice.
Author:
New Energy Development Organization
Year:
1987
Bibliographic source:
New Energy Development Organization, Tokyo

Materials and methods

Principles of method if other than guideline:
Determination of the lethal dose after intraperitoneal application of the test substance to monkeys in the context of a metabolism study.
GLP compliance:
not specified
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
monkey
Strain:
Macaca fascicularis
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Doses:
0, 25, 125, 600, 3000 mg/kg
No. of animals per sex per dose:
1 (a total of 5 animals was repeatedly used)
Control animals:
yes
Details on study design:
- Duration of observation period following administration: at least 2 days
- Frequency of observations and weighing: examination of clinical chemical parameters after 1, 3, 10, 24, 30, 48 hours
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, histopathology, other: clinical chemistry

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LDLo
Effect level:
3 000 mg/kg bw
Mortality:
During another part of the study 3 animals died after injection of 3000 mg/kg bw within 24 to 48 hours.
Clinical signs:
No abnormalitites were seen up to 600 mg/kg bw. All monkeys exposed to 3000 mg/kg fell into a coma immediately after administration and remained recumbent for 3 h to 2 d. Additionally, anorexia was observed.
Gross pathology:
No lesions in the eye, but slight effects in the liver.
Other findings:
- Histopathology: No lesions in the optic nerve or central nervous system
- Potential target organs: Liver
- Other observations: The highest dose level led to increased formate level, decreased pH in blood as well as delayed excretion of methanol and secondary products

Applicant's summary and conclusion