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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
N-acetylcysteine or trolox derivative mitigate the toxic effects of methanol on the antioxidant system of rat brain.
Author:
Farbiszewski, R. et al.
Year:
2000
Bibliographic source:
Toxicology 156: 47-55
Reference Type:
publication
Title:
Ethanol and N-acetylcysteine influence on the development of liver changes in experimental methanol intoxication.
Author:
Kasacka, I. and Skrzydlewska, E.
Year:
2001
Bibliographic source:
Roczniki Akademii Medycznej w Bialymstoku 46: 133-144
Reference Type:
publication
Title:
Protective effect of N-acetylcysteine on reduced glutathione, reduced glutathione-related enzymes and lipid peroxidation in methanol intoxication.
Author:
Skrzydlewska, E. and Farbiszewski, R.
Year:
1999
Bibliographic source:
Drug Alcohol Dep 57: 61-67
Reference Type:
publication
Title:
Trolox-derivative antioxidant protects against methanol-induced damage.
Author:
Skrzydlewska, E. and Farbiszewski, R.
Year:
1997
Bibliographic source:
Fund Clin Pharmacol 11 460-465

Materials and methods

Type of study / information:
Information on the effects of N-acetylcysteine or trolox derivative to mitigate the toxic potential of methanol on the antioxidant system of rats.
Principles of method if other than guideline:
Male Wistar rats were treated with N-acetylcysteine or trolox derivative during methanol intoxication to evaluate the antioxidant defense potential.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Methanol
EC Number:
200-659-6
EC Name:
Methanol
Cas Number:
67-56-1
Molecular formula:
CH4O
IUPAC Name:
methanol

Results and discussion

Any other information on results incl. tables

1. After oral application of methanol (3.0 g/kg body weight), the thiobarbituric acid-reactive substances indicating lipid peroxidation were significantly increased in brain tissue from 6 hours to 7 days after treatment. The activities of brain GSH peroxidase and GSH reductase were already reduced at 6 hours after administration. [Farbiszewski and Skrzydlewska, 2000] Also in liver, concentrations of lipid peroxidation products were increased and GSH peroxidase and GSH reductase levels were decreased. [Skrzydlewska and Farbiszewski, 1999]

2. N-Acetylcysteine exerted an protective antioxidant effect in the liver of methanol-treated rats: The total antioxidant status remained on the background-level after prior i.p. treatment with N-acetylcysteine (150 mg/kg body weight) [Farbiszewski and Skrzydlewska, 1999]. Furthermore it resulted in a lower degree of parenchymal damage [Kasacka and Skrzydlewska, 2001].

Concomitantly methanol-induced GSH-depletion was significant after 24 h and the content steadily decreased until 7 days. The toxic effects of methanol on the antioxidant system seems to be mitigated by N-acetylcysteine. In presence of N-acetylcysteine, enzyme activities involved in the removal of reactive oxygen species were also similar to the untreated control while significantly increased without protective counteraction. [Farbiszewski and Skrzydlewska, 2000].

3. A trolox derivative, an analog of alpha-tocopherol, is introduced as a beneficial antioxidant which prevents or reduces methanol-induced lipid peroxidation in rats [Skrzydlewska and Farbiszewski, 1997].

Applicant's summary and conclusion