Methanol

Additional toxicological data

Administrative data

Endpoint:

additional toxicological information

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

publication

Title:

N-acetylcysteine or trolox derivative mitigate the toxic effects of methanol on the antioxidant system of rat brain.

Author:

Farbiszewski, R. et al.

Year:

2000

Bibliographic source:

Toxicology 156: 47-55

Reference 2

Reference Type:

publication

Title:

Ethanol and N-acetylcysteine influence on the development of liver changes in experimental methanol intoxication.

Author:

Kasacka, I. and Skrzydlewska, E.

Year:

2001

Bibliographic source:

Roczniki Akademii Medycznej w Bialymstoku 46: 133-144

Reference 3

Reference Type:

publication

Title:

Protective effect of N-acetylcysteine on reduced glutathione, reduced glutathione-related enzymes and lipid peroxidation in methanol intoxication.

Author:

Skrzydlewska, E. and Farbiszewski, R.

Year:

1999

Bibliographic source:

Drug Alcohol Dep 57: 61-67

Reference 4

Reference Type:

publication

Title:

Trolox-derivative antioxidant protects against methanol-induced damage.

Author:

Skrzydlewska, E. and Farbiszewski, R.

Year:

1997

Bibliographic source:

Fund Clin Pharmacol 11 460-465

Materials and methods

Type of study / information:

Information on the effects of N-acetylcysteine or trolox derivative to mitigate the toxic potential of methanol on the antioxidant system of rats.

Principles of method if other than guideline:

Male Wistar rats were treated with N-acetylcysteine or trolox derivative during methanol intoxication to evaluate the antioxidant defense potential.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

Molecular formula:

CH4O

IUPAC Name:

methanol

Results and discussion

Any other information on results incl. tables

1. After oral application of methanol (3.0 g/kg body weight), the thiobarbituric acid-reactive substances indicating lipid peroxidation were significantly increased in brain tissue from 6 hours to 7 days after treatment. The activities of brain GSH peroxidase and GSH reductase were already reduced at 6 hours after administration. [Farbiszewski and Skrzydlewska, 2000] Also in liver, concentrations of lipid peroxidation products were increased and GSH peroxidase and GSH reductase levels were decreased. [Skrzydlewska and Farbiszewski, 1999]

2. N-Acetylcysteine exerted an protective antioxidant effect in the liver of methanol-treated rats: The total antioxidant status remained on the background-level after prior i.p. treatment with N-acetylcysteine (150 mg/kg body weight) [Farbiszewski and Skrzydlewska, 1999]. Furthermore it resulted in a lower degree of parenchymal damage [Kasacka and Skrzydlewska, 2001].

Concomitantly methanol-induced GSH-depletion was significant after 24 h and the content steadily decreased until 7 days. The toxic effects of methanol on the antioxidant system seems to be mitigated by N-acetylcysteine. In presence of N-acetylcysteine, enzyme activities involved in the removal of reactive oxygen species were also similar to the untreated control while significantly increased without protective counteraction. [Farbiszewski and Skrzydlewska, 2000].

3. A trolox derivative, an analog of alpha-tocopherol, is introduced as a beneficial antioxidant which prevents or reduces methanol-induced lipid peroxidation in rats [Skrzydlewska and Farbiszewski, 1997].