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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

With high probability acutely not harmful to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
15 400 mg/L

Additional information

In continuous flow-through systems LC50 (96 h) values for freshwater fish were 29400 mg/L (Pimephales promelas), 20100 mg/L (Salmo gairdneri), 15400 mg/L (Lepomis macrochirus) (Poirer, 1986), 20100 mg/L (Salmo gairdneri) and 28100 mg/L (Pimephales promelas) (Call, 1983). Reiff found in a study conducted under static conditions comparable toxicity values for rainbow trout (11850 mg/L). Since this result is based on nominal concentration, the value from the study of Poirer for Lepomis macrochirus (based on analytical measurements and conducted under flow-trough conditions) is taken as the lowest toxic concentration for freshwater fish. Toxicity and sublethal effects of methanol on swimming performance of the marine fish Florida pompano (Trachinotus carolinus) were investigated in a short-term experiment (24 h), resulting in LC50 > 10000 mg/L. The very low toxicity towards fish has been confirmed by the results of several studies investigating methanol as common cryoprotectant agent in early-life stages of fish (embryos, fish eggs, oocytes): Danio rerio (fish embryo toxicity test), EC50(48 h): 22100 mg/L (Lammer et al.); Danio rerio (OECD 212), NOEC(96h): 3950 mg/L (Chromcova et al.); Danio rerio (oocytes) NOEC (30 min): 64000 mg/L (Plachinta et al.).