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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
A toxicologic study of dimethyl sulfoxide
Author:
Willson JE, Brown DE and Timmens EK
Year:
1965
Bibliographic source:
Toxicol. Appl. Pharmacol. 7: 104-112

Materials and methods

Test guideline
Qualifier:
no guideline available
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
rat
Strain:
other: Carworth CFN
Sex:
male/female

Administration / exposure

Route of administration:
intravenous
Vehicle:
unchanged (no vehicle)
Details on exposure:
Each dose was administered over a 1-minute interval.
Doses:
2.5, 5.0, and 10 g/kg
No. of animals per sex per dose:
5 males and 5 females
Details on study design:
- Duration of observation period following administration: 14 days
Statistics:
Median lethal dose (LD50), 95% confidence limit, probit slopes and maximum tolerated dose (LD0.1) were determined.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
5 360 mg/kg bw
Mortality:
With one exception, deaths occurred within the first 24 hours
Clinical signs:
Death was preceded by tremors, myasthenia, dyspnea, and occassionally, convulsions
 Non-lethal doses of DMSO produced decreased motor activity and myasthenia

Applicant's summary and conclusion