Registration Dossier

Administrative data

Endpoint:
additional ecotoxicological information
Type of information:
experimental study
Adequacy of study:
other information

Data source

Reference
Reference Type:
publication
Title:
Acute toxicological effects of dimethyl sulfoxyde on the rough-skinned newt (Taricha granulosa)
Author:
Lappenbush WL and Willis DL
Year:
1971
Bibliographic source:
Toxicology and Applied Pharmacology, 18, 141-150

Materials and methods

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Any other information on results incl. tables

Species: amphibians

News were collected during the summer and spring, acclimated for 10 days at 10°C, weighed, treated with DMSO and maintained in an unfed state which simulated winter conditions.

Intraperitoneal injections of 15 g DMSO/kg or immersion in 2% DMSO were determined to be lethal threshold doses for newts. Death was rapid.

In addition to a transient increase in the body weight of the DMSO-treated animals, other changes were observed:
- a reduction in respiration to 70% that of the controls
- an increase in the weights of spleen (by day 1, the average spleen weight was over twice that of the controls), liver (33% increase after 24 h), and abdominal fat (150% increase after 20 days).

Lethality was attributed primarily to irreversible changes in the permeability of the skin, causing a water imbalance, hyperhydratation and death. The reduced respiratory rate increased the mortality rate

Applicant's summary and conclusion