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EC number: 200-664-3 | CAS number: 67-68-5
The toxic and cryoprotective effects of 10% DMSO concentration were studied in early stages of loach embryo development (stage 2(8 blastomeres) to stages 27-33 (organogenesis)). Embryos at stages 22-28 of development were most resistant to the toxic effects of DMSO. Maximum cryoprotection was observed at stages 25-33.The number of embryos which did not survive increased with decreasing temperature from 0 to -10 degree C.The number of embryos with developmental defects was greatest at -2 degree C.At lower temperatures, embryos died rather than developed abnormally.
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