Registration Dossier

Administrative data

Endpoint:
additional ecotoxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
up to 1985
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Documentation of study acceptable. Literature reference available.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1986
Report Date:
1985

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: see principles
Principles of method if other than guideline:
Control water preparation: high salinity brine (90%) was prepared by slow, gentle heating of local seawater (Narragansett Bay, RI) to obtain salt water of acceptable quality and low bacterial content. Dilution of brine to 30% with distilled water in the sea urchin tests.
Test samples: test compound was dissolved in diluted brine. Six to ten concentrations were used for determination of toxicity in the early embryo growth and the sperm cell tests, respectively.
Test procedures: Sea urchin embryo test: Sea urchin gametes were added to test solutions and exposed for 2h. 3-H-thymidine was added and incorporation allowed during another 2hrs of exposure. Embryos were collected, washed and processed on filters. Incorporation of 3-H-thymidine was measured by liquid scintillation counting and EC50 and 95%-C.I. determined. Sea urchin sperm cell test: Gametes were obtained from adults using electrical stimulation. Sperm concentrations were estimated spectrophotometrically at 540 nm after dilution to 1x10exp6 sperm/ml. Egg suspensions were counted microscopically and diluted to about 1,000/ml. Aliquots of sperm suspension (100 µl) were added to 10 ml of test solution, sperms exposed for 1 hr at 20°C and 1 ml of egg suspension was subsequently added to the test solution. Sperm:egg ratios were about 1,000:1. Fertilization (presence of fertilisation membranes) was determined by microscopic observation. Control fertilization rates were acceptable in a range of 60-90%. EC50 values and 95% C.I. were calculated by probit or moving average analyis.
Test comparison: Results from the early embryo growth and sperm cell toxicity test were compared with LC50 (96hr) and EC50 (48hr) literature values for acetylacetone.
GLP compliance:
not specified
Type of study / information:
Marine Toxicity tests in sea urchin (Arbacia punctulata)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data

Results and discussion

Any other information on results incl. tables

Comparison of test results of the two marine tests with results from standard tests in fresh water fish and water flea showed that acetyl acetone was more toxic to sperm cells while sensitivity of the early embryo test was comparable to the acute toxicity tests:

Early embryo test: EC50 = 105.4 mg/l (95% C.I. 56.5-170.3)
Sperm cell toxicity test: EC50 = 0.9 mg/l (95% C.I. 0.8-1.1)
LC50 (96hr, P. promelas) = 141.0 mg/l.
EC50 (48 hr, D. magna) = 47.6 mg/l

Applicant's summary and conclusion

Executive summary:

Comparing the results of the two marine tests with these from other tests in fresh water fish and water flea showed that acetylacetone was more toxic to sperm cells while sensitivity of the early embryo test was comparable to the acute toxicity tests