Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study with limited documentation

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
Principles of method if other than guideline:
The test was conducted in accordance with "Biodegradation test of chemical substance by microorganisms etc." stipulated in the Order
Prescribing the Items of the Test Relating to the New Chemical Substance (1974, Order of the Prime Minister, the Minister of Health and Welfare, the
Minister of International Trade and Industry No. 1). This guideline corresponds to "301C, Ready Biodegradability: Modified MITI Test (I)" stipulated in the OECD Guidelines for Testing of Chemicals (1981).
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Sludge concentration: 30 mg/l
Duration of test (contact time):
28
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
not reported
Reference substance
Reference substance:
not specified

Results and discussion

Preliminary study:
not reported
Test performance:
not reported, according to guideline
% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
83 - 100
Sampling time:
28 d
Details on results:
Indirect Analysis: BOD 83%
Direct Analysis: GC 100% ; TOC 95%

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Substance is readily biodegradable under the conditions used.
Executive summary:

A readily biodegradability test was performed with 2,4 -Pentanedione according to OECD 301C, Ready Biodegradability: Modified MITI Test (I). The concentration of the substance was 100 mg/l whereas the activated sludge concentration was 30 mg/l. The test showed a readily biodegradation of more than 80% after 28 days of exposure.