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EC number: 204-634-0 | CAS number: 123-54-6
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985-01-09 to 1985-04-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Conduction and documentation of study very acceptable. Study report available.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
- Reference Type:
- publication
- Title:
- The acute toxicity and primary irritancy of 2,4-pentanedione
- Author:
- Ballantyne, B., Dodd, D.E., Myers R.C., Nachreiner, D.J.
- Year:
- 1 986
- Bibliographic source:
- Drug and Chemical Toxicology, 9, 133-146
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as stated in report
- Deviations:
- no
- Principles of method if other than guideline:
- Method: other
5 male or 5 female Hilltop-Wistar rats (weight 200 - 300g) per group; 4 doses tested; 14 d postdosing observation period; undiluted TS was given by means of stomach intubation with a ball-end stainless steel needle. - GLP compliance:
- yes
- Test type:
- other:
- Limit test:
- no
Test material
- Reference substance name:
- Pentane-2,4-dione
- EC Number:
- 204-634-0
- EC Name:
- Pentane-2,4-dione
- Cas Number:
- 123-54-6
- Molecular formula:
- C5H8O2
- IUPAC Name:
- pentane-2,4-dione
- Details on test material:
- - Name of test material (as cited in study report): 2,4-Pentanedione
- Substance type: industrial chemical
- Physical state: liquid
- Analytical purity: > 99%
- Impurities (identity and concentrations): not reported
- Lot/batch No.: 496500
- Expiration date of the lot/batch: not given
- Stability under test conditions: stable
- Storage condition of test material: not given
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 200-300 g
- Fasting period before study: overnight
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): not given
- Humidity (%): not given
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): not reported
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: not used
MAXIMUM DOSE VOLUME APPLIED: 16 ml/kg bw - Doses:
- 1, 0.71, 0.50, 0.25 ml/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations daily, weighting at days 0, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- LD50s are calculated using the method of Thompson moving average method.
Results and discussion
- Preliminary study:
- not applicable
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 760 mg/kg bw
- 95% CL:
- 655 - 890
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 570 mg/kg bw
- 95% CL:
- 485 - 675
- Mortality:
- LD50 in male and female rats 760 and 570 mg/kg, respectively; most deaths occurred within 5 hours after administration
- Clinical signs:
- other: signs of toxicity included sluggishness, tremors, kyphosis, lacrimation, unsteady gait, comatose appearance and prostration. Survivors recovered at one to two days.
- Gross pathology:
- At necropsy, findings included few remarkable lesions except enlarged cervical lymph nodes in most animals, suggesting the presence of a minor infection.
- Other findings:
- not reported
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Substance is harmful to rats following oral exposure.
- Executive summary:
Acetylacetone (2,4 -Pentanedione) was administered to groups of 5 male and 5 female Hilltop-Wistar rats via peroral intubation. The rats received the test material (1, 0.71, 0.5 and 0.25 ml/kg bw respectively) by stomach intubation with a ball-endstainless steel needle. The sample was injected by means of a syringe and doses are varied by adjusting the volume of the test materials. The rats were fastened overnightbefore dosing.
The LD50 for male rats receiving 2,4 -Pentanedione were 760 mg/kg bw for males and 570 mg/kg bw for females. Sluggishness , tremors, kyphosis, lacrimation, unsteady gait, comatose appearance and prostration among the signs of toxicity noted. Most deaths occured within 5 hours. Survivors recovede at one to two days.
At necropsy findings included few remarkable lesions except enlarged cervial lymph nodes in most animals. This mostly slight condition suggested the presence of a minor infection. There were no clinical signs of such an infection and this condition was not believed to have affected the results of the test.
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