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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

2,4-Pentanedione was considered to be harmful to aquatic invertebrates based on the tests performed with Daphnia magna

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
25.9 mg/L

Additional information

Acetyl acetone was assessed for its toxicity to Daphnia magna in a static 48-hour immobilisation test in closed vessel design according to OECD guideline 202. Young Daphnia (< 24 hours old) were exposed in a static test to the test item for 48 hours, added to test medium at a range of concentrations. Under otherwise identical test conditions, concentrations of 100, 45, 21, 9.4 and 4.3 mg test item/L (nominal) and a controlresult in different percentages of Daphnia being no longer capable of swimming at the end of the test. 20 daphnids per control and test concentration, divided into 4 groups of 5 animals, each group in 110 mL test medium were used. After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 4.3 mg test item/L. At 9.4 mg test item/L one individual was immobile after 24 hours. However, as this occurred only in one replicate this effect is considered to be not test item related. At the concentration of 9.4 mg/L five animals in total were immobile, however three of them were immobile because they wereentangled in a particle. Their immobility was considered to be not test item related and was therefore not considered for statistical analysis.At 45 and 100 mg test item/L all daphnids were immobile after 48 hours of exposure. The EC50 is 25.9 mg/l. All validity criteria were met (Böttcher, IBACON 2010).

The acute toxicity of 2,4-pentanedione was also studied earlier in several static tests with Daphnia magna. The 48 h EC50-values obtained from these studies were 35.4 mg/l (nominal), 48 mg/l (measured) and 75 mg/l (nominal) thereby showing good comparability of all tests (Thurston et al. 1985, Mount and Norberg 1984, Bringmann and Kuehn 1982, Elnabarawy et al. 1986)

[modified from OECD SIDS Dossier].