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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

- key information: (48h) EL50 for Daphnia: >1000 mg/l  [OECD 202; test mat. (WAFs) (nominal) based on: mobility];
- supporting information: (48h) EL50 for Daphnia: >1000 mg/l [OECD 202; test mat. (WAFs) (nominal) based on: mobility]

Key value for chemical safety assessment

Additional information

The acute toxicity of a sample of the substance (Shell SMDS-2) has been determined by Palmer (2001) in tests with the freshwater invertebrate Daphnia magna (water flea). The test was conducted in accordance with OECD 202 methodology.

WAFs of the sample were prepared in sealed vessels with minimum headspace by stirring for approximately 72 hours. The contents of the vessels were left to stand for 1-2 hours before drawing off the aqueous phase – the WAF – for testing. Static exposures were then carried out in completely full, sealed vessels. The tests were not subject to GLP and the test media were not analysed for stability or for exposure concentration of the test substance. However in other respects they were considered to fulfil the requirements of current best practice.

The test results, expressed as the EL50value, showed that the sample was not acutely toxic to Daphnia magna at a loading rate of 1000 mg/l.

Albertus and Phillips (2005) have reported results for anacute toxicity test carried out ona second sample of the substance (SASOL SPD™ Gasoil)withthe freshwater invertebrate Daphnia magna (water flea). The test was conducted in accordance with OECD Test Guidelines 202. The test media were water-accommodated fractions of the test sample that were prepared in sealed vessels with a small headspace according to the CONCAWE methodology for the ecotoxicological testing of petroleum products (CONCAWE report, 1993).

The test results, expressed as the EL50value, showed that the sample was not acutely toxic to Daphnia magna at a loading rate of 1000 mg/l.

The following information is taken into account for short-term toxicity to aquatic invertebrates:

(48-h) EL50>1000 mg/l D. magna

Value used for CSA:

48-h EL50for freshwater invertebrates: >1000 mg/l.