Registration Dossier
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EC number: 481-740-5 | CAS number: 848301-67-7
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
OECD 211 (Daphnia magna, Reproduction Test):
- EL50(21-d): 32-100 mg/l (test mat. (WAF) (nominal) based on survival and reproduction;
- LOELR (21 d): 100 mg/l (test mat. (WAFs) (nominal) based on: survival and reproduction;
- NOELR (21 d): 32 mg/l (test mat. (WAFs) (nominal) based on: survival and reproduction.
Key value for chemical safety assessment
Additional information
A Daphnia reproduction study has been conducted with GTL Gasoil using the freshwater species Daphnia magna (Goodband, 2011). The test was conducted in accordance with OECD 211 and was compliant with GLP.
Daphnia magna were exposed to water accommodated fractions (WAFs) of the test material over a range of nominal loading rates of 10, 32 and 100 mg/l for a period of 21 days under semi-static test conditions. The test was conducted using completely full test vessels which were covered to minimise any potential for volatile losses of the test substance.
The 21-d EL50(immobilisation) value, based on nominal loading rates, for the parental Daphnia generation (P1) was between 32-100 mg/l loading rate WAF.
The 21-d EL50(reproduction) value, based on nominal loading rates was between 32-100 mg/l loading rate WAF.
The Lowest Observed Effect Loading Rate (LOELR) was considered to be 100 mg/l on the basis that at this loading rate, although no significant differences (P³0.05) were observed between the control and the 100 mg/l loading rate WAF test group in terms of numbers of live young produced per adult by Day 21, significant mortalities (immobilisation) were observed in the parental generation (P1).
The No Observed Effect Loading Rate (NOELR) was considered to be 32 mg/l on the basis that at this loading rate there were no significant mortalities (immobilisation) observed in the parental generation (P1) and that there were no significant differences (P³0.05) between the control and the 32 mg/l loading rate WAF test group in terms of numbers of live young produced per adult by Day 21.
Total Organic Carbon (TOC) analysis of the freshly prepared test preparations showed amount of carbon present within the 10, 32 and 100 mg/l loading rate WAF test vessels to range from less than the limit of quantitation (LOQ) for the method (1.0 mg carbon C/l) to 8.91 mg C/l. TOC analysis of the old test media showed amount of carbon present to range from less than the LOQ to 1.84 mg C/l.
The following information is taken into account for long-term toxicity to aquatic invertebrates:
(21-d) NOELR 32 mg/l [D. magna]
Value used for CSA:
21-d NOELR for freshwater invertebrates: 32 mg/l.
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