Methanesulphonic acid

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (lack of detailed documentation)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Reference 2

Reference Type:

review article or handbook

Title:

Methanesulfonic acid.

Author:

Shertzer HG

Year:

2001

Bibliographic source:

Patty's Toxicology, 5th edition, Vol 7, Ch. 94: 745-746

Reference 3

Reference Type:

publication

Title:

HPVIS, IUCLID Data Set for Methanesulphonic acid, CAS no. 75-75-2

Author:

US-EPA

Year:

2003

Bibliographic source:

HPV Chemicals Challenge Program OPPT NCIC 201-14249B

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

lack of detailed documentation

Principles of method if other than guideline:

Single administration of 0.1 ml of the test substance was placed in the conjuctival sac of one eye of each of two rabbits. The treated eye of one of the two animals was rinsed with flowing water (initiated 20 to 30 seconds after instillation and continued for one minute). The treated eye of the other animal was kept unwashed. The reaction was then scored periodically for 7 days.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methanesulphonic acid

EC Number:

200-898-6

EC Name:

Methanesulphonic acid

Cas Number:

75-75-2

Molecular formula:

CH4O3S

IUPAC Name:

methanesulfonic acid

Details on test material:

- Name of test material (as cited in study report): Methanesulfonic acid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eyes served as control

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 ml
- Concentration (if solution): undiluted (anhydrous solution)

Duration of treatment / exposure:

eye of one rabbit was washed out after 20 seconds and eye of another rabbit was not washed out

Observation period (in vivo):

7 days (the reaction was abserved at 10 min, 1h, 2h, 3h, 4h, 24h, 48h, 72h, 4 days, 5 days, 6 days and 7 days)

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: with flowing water for 1 minute (only one animal)
- Time after start of exposure: 20 seconds

SCORING SYSTEM: irritation responses were scored (no data on scoring system)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Remarks:

unwashed eye

Time point:

other: 24-48-72 h

Score:

4

Reversibility:

not fully reversible within: 21 days

Results 2

Irritation parameter:

chemosis score

Basis:

animal #1

Remarks:

unwashed eye

Time point:

other: 24-48-72 h

Score:

0

Reversibility:

other: no effects

Results 3

Irritation parameter:

cornea opacity score

Basis:

animal #2

Remarks:

washed eye

Time point:

other: 24-48-72 h

Score:

4

Reversibility:

not fully reversible within: 7 days

Results 4

Irritation parameter:

chemosis score

Basis:

animal #2

Remarks:

washed eye

Time point:

other: unwashed eye

Score:

0

Reversibility:

other: no effects

Irritant / corrosive response data:

Unwashed eye:
The reaction involved all ocular tissues and occured immediately. The conjunctivae became completely necrotic (white) without evident swelling. The iris was dilated with ragged edges and failed to react to light. The cornea opacified completely within 24 hours.

Washed eye:
The reaction did not differ significantly from that of the unwashed eye.

Other effects:

Each instillation caused excruciating pain.

Any other information on results incl. tables

Irritation scores:

Time	Unwashed eye				Washed eye
	Cornea	Iris	Redness	Chemosis	Cornea	Iris	Redness	Chemosis
10 min	2	2	N	0	2	2	N	0
1 h	2	2	N	0	2	2	N	0
2 h	2	2	N	0	4	?	N	0
3 h	2	2	N	0	4	?	N	0
4 h	2	2	N	0	4	?	N	0
24 h	4	?	N	0	4	?	N	0
48 h	4	?	N	0	4	?	N	0^D
72 h	4	?	N	0	4	?	N	0^D
4 days	4	?	N	0	4	?	N	0^D
5 days	4	?	N	0	4	?	N	0^D
6 days	4	?	N	0	4	?	N	0^D
7 days	4	?	N	0	4	?	N	0^D

N: total necrosis

D: discharge