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EC number: 200-898-6 | CAS number: 75-75-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Based on the pH value (< 2), the Methanesulphonic acid (MSA) is classified as corrosive to the skin, this is unterlined by in vitro (Corrositex) and in vivo test (mouse tail contact) indicating a corrosive potential GHS Cat 1B. Further an OECD 405 similar eye irriation test with rabbits shows servere irritating / corrosive effects.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation / corrosion
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 435 (In Vitro Membrane Barrier Test Method for Skin Corrosion)
- Principles of method if other than guideline:
- Corrositex continuous time monitor assay was used to detect the corrosive potential of the test substance. This assay is used as a standardized and quantitative in-vitro corrosivity test. The test is based on the time that is required for the test sample to pass through a biobarrier membrane and produce a change in the Chemical Detection System. Both anhydrous methane sulfonic acid (MSA) and 70 % MSA were tested.
- GLP compliance:
- no
- Species:
- other: in vitro Corrositex Assay
- Type of coverage:
- other: not applicable
- Vehicle:
- other: unchanged (no vehicle) for anhydrous MSA and water for 70% MSA
- Amount / concentration applied:
- TEST MATERIAL
- Concentration (if solution): anhydrous and 70 % MSA - Duration of treatment / exposure:
- 4 hours, single exposure
- Observation period:
- 4 hours
- Number of animals:
- not applicable
- Details on study design:
- Corrositex Assay:
- The Corrositex Biobarrier Membranes consist of a reconstituted collagen matrix was placed onto the CDS solution contained in test vials. The biobarrier membrane batch was qualified by testing the positive control (NaOH), a blank vial serves as negative control. Prior to testing a qualification screen and a categorization screen are performed to validate if the assay is appropriate for testing of the substance and to categorize the test substance as weak or strong acid/base, respectively.
- For testing, 500 µl of the test substance are placed on the membrane, 4 replicates are prepared and tested at the same time.
- Once the test substance is placed on the membrane disc, the vials are continuously observed for the first 10 minutes and then at approximately 5-minute intervals for up to 4 hours. The elapsed time from test substance application until CDS colour change is recorded.
SCORING SYSTEM:
Time required for CDS (chemical detection system) colour change (minutes):
0 to 3 min: corrosive (Packing Group I, GHS Cat 1A)
> 3 to 60 min: corrosive (Packing Group II, GHS Cat 1B)
> 60 to 240 min: corrosive (Packing Group III, GHS Cat 1C)
> 240 min: non-corrosive - Irritant / corrosive response data:
- Average break-through time (mean of 4 determinations): 7 min 02 s (70 % methane sulfonic acid) and 3 min 43 s (anhydrous).
Reference
Test Article | Break Through Time (min:sec) | |||||||
Sponsor's designation | MA Number | Vial 1 | Vial 2 | Vial 3 | Vial 4 | Mean | Packing group | pH* |
70 % Methane Sulfonic Acid | 95BW22 | 7:20 | 7:00 | 6:50 | 6:57 | 7:02 | II | 0 |
Methane Sulfonic Acid | 95BW23 | 3:50 | 3:38 | 3:50 | 3:35 | 3:43 | II | 0 |
NaOH | - | 12:23 | NA | NA | NA | 12:23 | NA | NA |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (corrosive)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (lack of detailed documentation)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- lack of detailed documentation
- Principles of method if other than guideline:
- Single administration of 0.1 ml of the test substance was placed in the conjuctival sac of one eye of each of two rabbits. The treated eye of one of the two animals was rinsed with flowing water (initiated 20 to 30 seconds after instillation and continued for one minute). The treated eye of the other animal was kept unwashed. The reaction was then scored periodically for 7 days.
- GLP compliance:
- no
- Species:
- rabbit
- Strain:
- not specified
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated eyes served as control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 ml
- Concentration (if solution): undiluted (anhydrous solution) - Duration of treatment / exposure:
- eye of one rabbit was washed out after 20 seconds and eye of another rabbit was not washed out
- Observation period (in vivo):
- 7 days (the reaction was abserved at 10 min, 1h, 2h, 3h, 4h, 24h, 48h, 72h, 4 days, 5 days, 6 days and 7 days)
- Number of animals or in vitro replicates:
- 2
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing: with flowing water for 1 minute (only one animal)
- Time after start of exposure: 20 seconds
SCORING SYSTEM: irritation responses were scored (no data on scoring system) - Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Remarks:
- unwashed eye
- Time point:
- other: 24-48-72 h
- Score:
- 4
- Reversibility:
- not fully reversible within: 21 days
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- unwashed eye
- Time point:
- other: 24-48-72 h
- Score:
- 0
- Reversibility:
- other: no effects
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Remarks:
- washed eye
- Time point:
- other: 24-48-72 h
- Score:
- 4
- Reversibility:
- not fully reversible within: 7 days
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Remarks:
- washed eye
- Time point:
- other: unwashed eye
- Score:
- 0
- Reversibility:
- other: no effects
- Irritant / corrosive response data:
- Unwashed eye:
The reaction involved all ocular tissues and occured immediately. The conjunctivae became completely necrotic (white) without evident swelling. The iris was dilated with ragged edges and failed to react to light. The cornea opacified completely within 24 hours.
Washed eye:
The reaction did not differ significantly from that of the unwashed eye. - Other effects:
- Each instillation caused excruciating pain.
Reference
Irritation scores:
Time |
Unwashed eye |
Washed eye |
||||||
Cornea |
Iris |
Redness |
Chemosis |
Cornea |
Iris |
Redness |
Chemosis |
|
10 min |
2 |
2 |
N |
0 |
2 |
2 |
N |
0 |
1 h |
2 |
2 |
N |
0 |
2 |
2 |
N |
0 |
2 h |
2 |
2 |
N |
0 |
4 |
? |
N |
0 |
3 h |
2 |
2 |
N |
0 |
4 |
? |
N |
0 |
4 h |
2 |
2 |
N |
0 |
4 |
? |
N |
0 |
24 h |
4 |
? |
N |
0 |
4 |
? |
N |
0 |
48 h |
4 |
? |
N |
0 |
4 |
? |
N |
0D |
72 h |
4 |
? |
N |
0 |
4 |
? |
N |
0D |
4 days |
4 |
? |
N |
0 |
4 |
? |
N |
0D |
5 days |
4 |
? |
N |
0 |
4 |
? |
N |
0D |
6 days |
4 |
? |
N |
0 |
4 |
? |
N |
0D |
7 days |
4 |
? |
N |
0 |
4 |
? |
N |
0D |
N: total necrosis
D: discharge
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Additional information
The available information from acute dermal toxicity testing and on the pH value (<2) meet already the criteria for classification of MSA as corrosive to the skin, therefore no further animal test is suggested.
Nevertheless, the further available information shows that MSA is corrosive to biomembranes based on the results of Corrositex continuous time monitor assay (ElfAtochem 1995). This assay is used as a standardized and quantitative in-vitro corrosivity test. The test is based on the time that is required for the test sample to pass through a biobarrier membrane and produce a change in the Chemical Detection System. Both anhydrous MSA and 70 % MSA were tested. The average break-through time (mean of 4 determinations) were found to be 7 min 02 s (70 % methane sulfonic acid) and 3 min 43 s (anhydrous) indicating corrosive potential (GHS Cat 1B; EU R34). This result is furthermore underlined by a less reliable 1h mouse tail contact study showing corrosive results (Pennwalt 1976).
The eye irritation potential was investigated in an old and lower reliable OECD 405 similar study with rabbits (Pennwalt 1978). As servere cornea effects were observed (grading 4) and the effects were not reversible within 7 days the substance was to be servere irritating / corrosive to eye.
Summarising MSA is corrosive to skin GHS, CAT 1B.
Effects on skin irritation/corrosion: corrosive
Effects on eye irritation: corrosive
Justification for classification or non-classification
There is sufficient information available which warrant classification of methanesulfonic acid as corrosive to the skin. According to Annex VI to the CLP regulation (EC) 1272/2008 the substance meet criteria for C, R34 and Category 1B according to GHS criteria. Classification as corrosive implies also serious eye damage.
Due to the corrosive character which causes the toxic effect an irritation of respiratory system can not be excluded when inhaled. Therefore MSA is further classified as STOT SE 3, H335: May cause respiratory irritation.
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