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Acute tests on all three trophic levels were performed to examine the aquatic toxicity of 1.2 -epoxybutane. Daphnia turned out to be the most sensitive species revealing an EC50 (48h) of 70 mg/L. Thus, 1.2 -epoxybutane is considered to be acutely harmful to aquatic organisms. Effects of

1.2 -epoxybutane towards fish and algae were found in concentrations above 100 mg/L.

No data on long-term toxicity on fish and Daphnia are available. The hazard assessment of 1.2 -epoxybutane reveals neither a need to classify the substance as dangerous for the environment, nor is it a PBT or vPvB substance. Therefore, and for reasons of animal welfare, a long-term toxicity study in fish and Daphnia is not provided.

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