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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
3 September 1997 - 17 September 1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: Eathworm
- Source: Earthworms were obtained from Mrs H. Baker, Brickyard Farm, Buckworth Road, Alconbury Weston, Cambridgeshire, England.

ACCLIMATION
- Acclimation period: 20 days
- Health during acclimation (any mortality observed): no mortalities
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Test temperature:
23 to 25°C
pH:
approximately 6.0
Moisture:
35% of the dry weight of soil
Details on test conditions:
TEST SYSTEM

- Composition (if artificial substrate):
Industrial quartz sand 70% w/w
Kaolin clay 20%w/w
Sphagnum peat 10% w/w


OTHER TEST CONDITIONS
- Photoperiod: Continuous lighting
- Light intensity: 700 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
On Days 7 and 14 the medium was emptied from the test containers and the worms removed for counting. Mortalities live worms and those unaccounted for were recorded. Mortality was assessed by testing the reaction of each worm to a mechanical stimulus at either end. On Day 7 the medium was returned to the containers and surviving worms replaced on the surface.


VEHICLE CONTROL PERFORMED: yes


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Test concentrations: Dose levels based on study guidelines.

Nominal and measured concentrations:
Nominally 0 (control), 95, 171, 309, 556 and 1000 ppm.
Reference substance (positive control):
yes
Remarks:
chloroacetamide
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
1 000 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: Seven mortalities occurred during the study but were not considered to be related to treatment
- Total mass of adults at beginning of test: 300 - 600 mg per worm
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: All groups showed a decrease in bodyweight over the study A small weight loss is not unusual and frequently occurs in studies ofthis type Notreatment related trend was evident
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: Day 14 LC50 46 ppm
Validity criteria fulfilled:
no
Conclusions:
Under the conditions of this study the LC50 value of DPGDB to the earthworm was found to be in excess of 1000 ppm. The no observed effect level (NOEL) was considered to be 1000 ppm.
Executive summary:

An acute toxicity study to earthworms was conducted to determine the effect of the test material, DPGDB. The study was conducted according to OECD and EU test guidelines, and in compliance with GLP.

Groups of earthworms were exposed to DPGDB at levels 0 (control), 95, 171, 309, 556 and 1000 ppm in an artificial standard soil, for a test period of 14 days. Each treatment and control group consisted of four replicate containers, each with ten worms per container (forty worms per group, total).

No mortalities were seen during the study, and all worms were normal in appearance on days 7 and 14 of the test.. Under the conditions of this study, the LC50 value of DPGDB to the earthworm was found to be in excess of 1000 ppm. The no observed effect level (NOEL) was considered to be 1000 ppm.

Description of key information

In an acute toxicity study to earthworms conducted to determine the effect of DPGDB (HLS 1998, VCL 259/972156), no mortalities were seen during the study, and all worms were normal in appearance on days 7 and 14 of the test. Under the conditions of this study, the LC50 value of DPGDB to the earthworm was found to be in excess of 1000 ppm. The no observed effect level (NOEL) was considered to be 1000 ppm.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw

Additional information