Diisodecyl azelate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 - 22 Oct 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Analytical purity not stated.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solution was prepared according to the national standard procedure (DEV S4): 10 g of the test substance was added to 1 L deionised water and shacked overhead for 24 h. The solution was filtered with a folded filter. The filtered solution was added directly to the test vessels.

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: golden orfe
- Source: Fischzucht Eggers, Hohenwestedt, Germany
- Age at study initiation: approx. 4 weeks
- Corpulence factor: 0.8 - 1.1 g/cm3


ACCLIMATION
- Acclimation conditions (same as test): yes
- Type and amount of food: food directly from the supplier of the animals
- Feeding frequency: 2 times a week

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

15 °dH

Test temperature:

20 ± 1 °C

pH:

8.3 - 8.6

Dissolved oxygen:

7.4 - 9.2 mg/L

Nominal and measured concentrations:

Nominal concentration: 10000 mg/L filtered test solution

Details on test conditions:

TEST SYSTEM
- Material, size, fill volume: glass, 28.5x21.0x24.0 cm, 10 L
- Aeration: slightly aerated
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Drinking water from Cologne, Germany mixed with deionised water.

EFFECT PARAMETERS MEASURED: Mortality was recorded after 24, 48, 72, 96 h test duration.

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: Because the test substance is poorly water soluble the filtrate from a stock solution of 10 g/L was used to analyse if there are any effects in the range of water solubility.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 10 g/L

Nominal / measured:

nominal

Conc. based on:

other: filtered test solution

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

other: filtered test solution

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: 0%

Sublethal observations / clinical signs:

No effects of the test substance in the range of water solubility were observed testing a saturated test solution which was subsequently filtered. Thus, an LC50 (96 h) greater then the highest tested concentration of 10 g/L is determined.

Description of key information

No toxic effects up to the limit of water solubility (< 0.05 mg/L in aqua dest) for Leuciscus idus (OECD 203).

Additional information

One study investigating the toxicity of Diisodecyl azelate (CAS 28472-97-1) to the orfe Leuciscus idus melanotus is available (Institut für gewerbliche Wasserwirtschaft und Luftreinhaltung e.V., 1993). The test substance is described as an unknown, variable composition, complex reaction product or biological material (UVCB) under REACH. This study was conducted according to OECD guideline 203 in a limit test with 10 g/L filtered test solution. No mortality or other adverse effects were reported after 96 hours exposure to the test substance, thus the LC50 (96 h) is greater than 10 g/L. Therefore, no toxicity within the range of water solubility of the test substance was observed.