Registration Dossier
Registration Dossier
Diss Factsheets
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EC number: 208-796-3 | CAS number: 542-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- There is no data available on skin sensitation studies with Acetoguanamine. According to Annex VII of REACH regualtion before new tests are carried out to determine the listed enpoints of this Annex all available in-vitro data, in-vivo data, historical human data, validated (Q)SAR-data and data from structurally related substances (read-across approach) shall be assessed first. Therefore a literatur research was performed to find in-vitro data, in-vivo data or historical human data on skin sensitation with Acetoguanamine. No records were found and in order to avoid unnecessary animal testing structurally related substances were searched. A structurally related substance is Benzoguanamine, for which data on skin sensitation was found. Because of the fact that a read-across approach is always endpointspecific it was evaluated with the help of the "endpoint specific guidance" of ECHA in chapter R.7.3 ("skin and respiratory sensitisation") whether a read across between Benzo- and Acetoguanamine is possible. According to this guidance "the skin sensitisation potential of a chemical is related to its ability to react with skin proteins to form covalently linked conjugates and recognition of these by the immune system. In the vast majority of cases, this is dependent on electrophilic reactivity of the skin sensitizer or a derivative produced (usually by oxidation) in vivo or abiotically (Barratt et al., 1977)." The guidance also states the most frequently encountered types of electrophile-nucleophile reactions in skin sensitisation: "Michael-type reactions, SN2 reactions, SNAr reactions, acylation reactions and Schiff-base formation. These chemical reaction mechanisms can serve as a means of describing the domain of applicability (the scope) of (Q)SAR or form the basis for grouping chemicals into chemical categories. " Regarding the structure of Benzo- and Acetoguanamine it can be assumed, that both substances show a likely chemical reactivity in the mentioned electrophile-nucleophile reactions and therefore a simaliar skin sensitising effect can be assumed. This is supported by similar physico-chemical parameters of both substances. According to the above mentioned guideline "although the main factors driving skin sensitation (and therefore the (Q)SAR) is the underlying premise of the electrophilicity of a chemical, other factors such as hydrophobicity encoded in the octanol/water partition coefficient (log P) may also be considered as playing a role in the modifying the sensitisation response observed." Aceto- and Benzoguanamine both have a logPow in the same range (logPow(Acetoguanamine)= -0.88; logPow(Benzoguanamine)=1.38), which means that they might show a likely skin penetration ability.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 6-phenyl-1,3,5-triazine-2,4-diyldiamine
- EC Number:
- 202-095-6
- EC Name:
- 6-phenyl-1,3,5-triazine-2,4-diyldiamine
- Cas Number:
- 91-76-9
- IUPAC Name:
- 6-phenyl-1,3,5-triazine-2,4-diamine
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source:Harlan Laboratories, NL
- Age at study initiation: 8-12 weeks
- Weight at study initiation: 15-25 g
- Housing: group housing (Makrolon Type III)
- Diet (e.g. ad libitum): pelleted standard diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2 °C
- Humidity (%): 45-65 %
- Air changes (per hr): no information available
- Photoperiod (hrs dark / hrs light): 12 / 12
Study design: in vivo (LLNA)
- Vehicle:
- dimethyl sulphoxide
- Concentration:
- 2.5%, 5%, 7.5%
- No. of animals per dose:
- 4
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: Group 1 (control group): 1.00 Group 2 (2.5%): 1.44 Group 3 (5%): 1.11 Group 4 (7.5%): 1.09
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: Group 1 (control group): 225.6 Group 2 (2.5%): 325.5 Group 3 (5%): 251.0 Group 4 (7.5%): 245.5
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- The presented guideline study is reliable and adequate for the chemical safety assessment of 6-phenyl-1,3,5-triazine-2,4-diyldiamine. Based on the findings of the study the test substance is classified as not sensitising.
- Executive summary:
There is no data available on skin sensitation studies with Acetoguanamine. In order to avoid unnecessary animal testing and to fullfill the claim of REACH regulation Annex VII ((Q)SAR-data and data from structurally related substances (read-across approach) shall be assessed first before new tests are carried out) related substances were searched.
A structurally related substance is Benzoguanamine, for which data on skin sensitation was found. The read-across approach was evaluated by the help of the "endpoint specific guidance" of ECHA in chapter R.7.3 ("skin and respiratory sensitisation"). For the evaluation see above in "Rationale for reliability".
The presented study according to OECD 429 ("Skin Sensitation: Local Lymph Node Assay") with Benzoguanamine is adequate for the chemical safety assessment. Based on the findings of this study no classification as sensitising is needed.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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