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In accordance with column 2 of REACH Annex IX, the effects on terrestrial soil organisms (macroorganisms, microorganisms, and plants) studies do not need to be conducted as direct and indirect exposure of the soil compartment with Acetoguanamine is unlikely. The substance is only used at industrial sites. Therefore an application to soil is not an identified use of the substance (CSR section 2.2) and in addition, it does not exist an indirect application of the substance to soil via sewage sludge. Furthermore due to its low log Kow (log Kow = - 0.88) the substance has a low adsorptive as well as a low bioaccumulative potential. Hence, a relevant distribution into soil and a considerable exposure of soil organisms is not expected.