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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This study has GLP and Quality assurance statements approved by China authorities

Data source

Reference Type:
study report
Report Date:

Materials and methods

Test guideline
according to
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes (incl. certificate)
The statements (GLP, Quality assurance) are part of the report

Test material

Details on test material:
Batch: T91021
Solubility in water: <1mg/L
Log Pow 8.6-9.7
Purity: Not applicable

Sampling and analysis

Analytical monitoring:
not required

Test substrate

Details on preparation and application of test substrate:
Since the solubility of test substance is lower than 1mg/L in water, the test substance was weighed with a balance and mixed with silica sand. The mixture was mixed thoroughly with basic test substrates and then with deionized water to obtain an even distribution of the test substance in the test substrates.

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
Details on test organisms:
The study was performed with earthworms (Eisenia foetida). This is the species recommended in Ministry of Environmental Protection of China, the Guildelines for the Testing of Chemicals, 2004, 207 Earthworm, Acute Toxicity Test. Ease of culturing and handling, sensitivity to a variety of chemical substances, and the extensive database for this species make them suitable for acute toxicity testing.
The test was performed with Eisenia foetida which were purchased from Shanghai Yinquan Agriculture Development Co., Ltd. (ID: EF-MKT-100930). All newly acquired earthworms were quarantined and observered for 14 days prior to use in the test. Earthworms were bred in cow dung and kept under 20±2°C for 7 days prior to testing. At the start of the test, the used adult earthworms, which were originated from the same population, were with clitellum and body weight of 300 to 600 mg.

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
Total exposure duration:
7 d
A range-finding test were carried out at 10, 100 and 1000 mg/kg dry artificial soil weight as well as a blank control. 10 earthworms were used for each concentration of treatment and contro
Post exposure observation period:
Based on result of range-finding test, one treatment group which nominal concentration was 1000 mg/kg dry artificial soil weight and one control group were conducted in definitive test. 40 earthworms were used for both groups, divided into four replicates of ten earthworms each.

Test conditions

Test temperature:
between 20.2-21.4°C
6.84 (at the beginning of the test)
The moisture of artificial soil were from 35.9-38.7% dry soil weight
Details on test conditions:
1L glass breakers were used as the test vessels. The test substrate for each concentration was divided into four equal quantities of 750 g as determined by weight. Each portion was placed into a separate glass beaker and represented one replicate for exposing 10 earthworms at the same concentration.

The earthworms, which were washed quickly with surplus water absorbed on filter paper before use, were randomly separated into groups of 10. These groups were weighed and then randomly assigned to the beakers by placed on the test substrate surface. The beakers were covered with film to prevent the substrate drying and were kept in artificial climate chamber for 14 d.

Each beaker was checked for dead and observations recorded at 7 and 14 d after the beginning of the test. The test substrates were spreaded on glass plate. The earthworms were examined and their reaction to a gentle mechanical stimulus were tested. Earthworms were considered dead if they did not respond to a gentle mechanical stimulus. Missing earthworms were also considered dead. Any adverse effects were recorded. When the examinaton was made at 7 d, the beaker was refilled with the substrate and the surviving earthworms were replaced on the same test substrate surface.

The moisture and temperature of the test substrates were measured at the beginning and the end of the test in a beaker of control group.
pH of the test substrates was measured at the beginning of the test in control group.
Nominal and measured concentrations:
The nominal concentration was 1000 mg/kg

Results and discussion

Any other information on results incl. tables

In range-finding test, no mortality was observed in all three treatment groups

Mortalities in control group and treatment group were 0 on 7th and 14th day

The 7 days and 14 days LC50value of the test substance toEisenia foetidawere higher than 1000 mg/kg dry soil weight

At the end of the test, the average body weight of the surviving earthworms in control group was significantly decrease(p<0.05). However, there were no significant differents (p>0.05) in the average body weight of the surviving earthworms between control group and treatment group

Applicant's summary and conclusion

Expressed as the nominal concentration of the test substance, the lethal effect of SENSANOV WR (SPQP20101202-0165) on Eisenia foetida is as following:
The 7 days LC50 value was higher than 1000 mg/kg dry soil weight.
The 14 days LC50 value was higher than 1000 mg/kg dry soil weight.
Executive summary:

Expressed as the nominal concentration of the test substance, the lethal effect of SENSANOV WR (SPQP20101202-0165) on Eisenia foetida is as following: The 7 days LC50 value was higher than 1000 mg/kg dry soil weight. The 14 days LC50 value was higher than 1000 mg/kg dry soil weight.