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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
BPL 11-0012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This study has its good laboratory compliance statement signed on September 9th 2011 (SGS)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report Date:
2011

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
Principles of method if other than guideline:
The amendement has been performed because initial way of solubilisation wasn't appropriated
GLP compliance:
yes (incl. certificate)
Remarks:
the GLP and Quality assurance statements have signed by SGS Multilab

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
The test material is exactly the registered substance

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
Activated sludge was centrifuged at 1000 rpm during 5 min and the supernatant was eliminated. Then, fresh media was added, mixed and centrifuged one more time. the supernatant was eliminated and fresh media was added. 5 mL of final sludge was filtered and dried to determine suspended mater. The result was as follow:

Filter and crucible = 2.13602 g
Volume of sludge = 5 mL
Final total dry wait = 2.2285 g

(2.2285 – 2.13602) / 0.005 = 18.496 g/l

To obtain final sludge concentration of 30 mg/L in reaction vessel we have inoculated 2L of media with 3.2 mL of this mixture
Duration of test (contact time):
>= 28 - < 29 d
Initial test substance concentration
Initial conc.:
10 mg/L
Based on:
other: TOC (test item) Total Organic Carbon introduced in essay vessel
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Remarks:
CO2 produced by microbial activities were determined by titration of barium bubbling with hydrochloric acid 0.05M and phenolphthalein. For 100ml of Ba(OH)2 0.0125 M, CO2 produced were calculated as follow : CO2 (mg) = 1.1 x (50 – HCl vol (ml))
Details on study design:
As follow the design:
- 2 bottles with test media and inoculum (control),
- 2 bottles with test media, inoculum and reference item (sodium benzoate) (reference test),
- 2 bottles with test media, inoculum and test item to have a concentration from 10 mg/L of TOC (test item solution),
- 1 bottle with test media, inoculum, test item and reference item (sodium benzoate) (toxicity test).
-3 bottles with test media, inoculum and test item water emulsion to have a concentration from 10 mg/L of TOC (test item solution),
- 1 bottle with test media, inoculum, test item water emulsion and reference item (sodium benzoate) (toxicity test).


Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
ca. 40
Sampling time:
28 d

Applicant's summary and conclusion

Interpretation of results:
other: This key study, shows clearly that SENSANOV WR deserved the more the qualification of inherently than not biodegradable.
Conclusions:
Test item is not readily biodegradable according to the OECD criteria.
Executive summary:

 

LCE11034

 Batch T02411, Product 36500Z

 

BPL11-0012.001

Percentage of biodegradation at 28 days

44 %

Conclusion

Not Readily Biodegradable