Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August -September 2008
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): LCE07105
- Substance type: UVCB
- Physical state: SOLID, white flakes
- Composition of test material, percentage of components: Alcohol C20/22 40-50% and Alkylpolyphosphate 50-60%
- Lot/batch No.: T71241
- Storage condition of test material: at room temperature in the dark
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 70-75°C
- Boiling point: >300°C
- Vapour pressure: 6.66 x 10-5 Pa.
- Water solubility (under test conditions): <1mg/L



OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: not considered as ready biodegradable

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
21 +-1°C
pH:
7.6 to 7.9
Dissolved oxygen:
2.2 to 2.5 mg/L
Nominal and measured concentrations:
1000mg/L
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
meas. (initial)
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Results with reference substance (positive control):

The reference material gave a 3 -Hour EC50 value of 8.6mg/L, 95% confidence limits 6.7 -11mg/L.

Any other information on results incl. tables

A study was performed to asses the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD guidelines for testing of chemicals (1984) N° 209 "activated sludge, inhibition respiration test" method C11 of EEC commission directive 88/302/EEC and US EPA drafts ecological effects test guidelines OPPTS 850.6800.

Following a preliminary range finding test, activated sewage sludge was exposed to an aqueous dispersion of the test material at a concentration of 1000mg/L (three replicate flasks) for a period of 3 hours at a temperature of approximately 21°C with the addition of a synthetic sawage as respiratory substrate.

The rate of respiration was determined after 30 minutes and 3 Hours contact time and compared to data for the control and a reference material 3,5 dichlorophenol.

The result of the test material on the respiration of activated sewage sludge gave a 3-hours EC50 of greater than 1000mg/L. The No Observed Effect Concentration (NOEC) after 3 Hours exposure was 1000mg/L.

It was considered unnecessary and unrealistic to test at concentrations in excess of 1000mg/L.

The reference material gave a 3 -Hour EC50 value of 8.6mg/L, 95% confidence limits 6.7 -11mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The effect ot the test material on the respiration of activated sewage sludge micro-organisms gave an 3-hour EC50 of greater than 1000mg/L. the No Observed Effect Concentration after 3 hours of exposure was 1000mg/L.
Executive summary:

A study was performed to asses the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD guidelines for testing of chemicals (1984) N° 209 "activated sludge, inhibition respiration test" method C11 of EEC commission directive 88/302/EEC and US EPA drafts ecological effects test guidelines OPPTS 850.6800.

Following a preliminary range finding test, activated sewage sludge was exposed to an aqueous dispersion of the test material at a concentration of 1000mg/L (three replicate flasks) for a period of 3 hours at a temperature of approximately 21°C with the addition of a synthetic sawage as respiratory substrate.

The rate of respiration was determined after 30 minutes and 3 Hours contact time and compared to data for the control and a reference material 3,5 dichlorophenol.

The result of the test material on the respiration of activated sewage sludge gave a 3-hours EC50 of greater than 1000mg/L. The No Observed Effect Concentration (NOEC) after 3 Hours exposure was 1000mg/L.

It was considered unnecessary and unrealistic to test at concentrations in excess of 1000mg/L.

The reference material gave a 3 -Hour EC50 value of 8.6mg/L, 95% confidence limits 6.7 -11mg/L.